Investigation on extracellular lipase production by Aspergillus japonicus isolated from the paper nest of Ropalidia marginata

A  Jayaprakash and P  Ebenezer

doi:10.17485/ijst/2010/v3i2.7

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Investigation on extracellular lipase production by Aspergillus japonicus isolated from the paper nest of Ropalidia marginata

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2010/v3i2.7

Year: 2010, Volume: 3, Issue: 2, Pages: 113-117

Original Article

Investigation on extracellular lipase production by Aspergillus japonicus isolated from the paper nest of Ropalidia marginata

A. Jayaprakash and P. Ebenezer ^*

Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
[email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Aspergillus japonicus isolated from the paper nest of Ropalidia marginata was screened for its extracellular lipase production. Maximum lipase activity (120 U/ml) was achieved by manipulating the culture environment. The optimum pH was 7.5 and the temperature was 40°C. Sucrose served as the best carbon source whereas peptone was the best nitrogen source for lipase production. An animal fat, pig fat in the culture medium enhanced lipase production. These investigations will be helpful in further scaling-up processes of this industrial enzyme. Owing to many applications in high-value syntheses and as bulk enzymes, lipases have high impact on bioprocessing.
Keywords: Aspergillus japonicus; paper nest; pig fat; Ropalidia marginata; lipase; Tween-20.