Molecular Phylogeny and Genetic Analysis of Green Leafhopper - Nephotettix virescens (Distant) using Mitochondrial COI Gene

K  Sreejith and C  D  Sebastian

doi:10.17485/ijst/2015/v8i1/52842

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Molecular Phylogeny and Genetic Analysis of Green Leafhopper - Nephotettix virescens (Distant) using Mitochondrial COI Gene

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2015/v8i1/52842

Year: 2015, Volume: 8, Issue: 1, Pages: 61–64

Original Article

Molecular Phylogeny and Genetic Analysis of Green Leafhopper - Nephotettix virescens (Distant) using Mitochondrial COI Gene

K. Sreejith and C. D. Sebastian^*

Division of Molecular Biology, Department of Zoology, University of Calicut, Kerala. 673635, India; drcdsebastian@gmail.com

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Accurate and rapid diagnosis of taxa, is of crucial importance to quarantine, pest management and analysis. Advances in DNA technology has resulted in an exceedingly vary of molecular techniques which might be employed in phylogenetically focussed approaches. Use of DNA sequence data derived from region of mitochondrial cytochrome oxidase I gene (mtCOI) has been widely used to estimate phylogenetic relationships at different taxonomic levels across insects. The present study investigates the molecular evolution of the Nephotettix virescens using COI gene (DDBJ accession No. AB976528; ENA accession No. LM994675) and its usefulness for reconstructing phylogenetic relationships within and among the leafhopper species.

Keywords:
DNA Sequencing, Leafhopper, Molecular Evolution, Mitochondrial COI, Nephotettix virescens, Phylogenetics