• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2023, Volume: 16, Issue: 45, Pages: 4123-4130

Original Article

Oleic acid from Marine seaweed Caulerpa scalpelliformis induced toxicity in Oreochromis niloticus

Received Date:17 September 2023, Accepted Date:02 November 2023, Published Date:05 December 2023


Background/Objective: Oleic acid is an unsaturated fatty acid, abundant in plants and animals. It accounts for 49% to 83% of total fatty acids, and altered oleic acid leads to diabetes, liver damage, and atherosclerosis. Alteration in oleic acid consumption leads to hepatotoxicity in humans. Macro algae named Caulerpa scalpelliformis is well-known for its bioactivity properties like antimicrobial and anti-cancer activity. Oreochromis niloticus (Nile tilapia) is a freshwater breed considered a protein-rich fish also it has a fast-growing capacity, easy farm management, and pleasant flavor with fewer bones, used as a study tool in scientific research. This study aims to determine the toxic effect in O. niloticus by inducing oleic acid from seaweed C. scalpelliformis. Methods: In the present study, oleic acid was extracted from C. scalpelliformis by column chromatography and analyzed using Thin Layer Chromatography (TLC). Toxicity analysis in O. niloticus was performed by exposing the C.scalpelliformis methanol extract in a freshwater fish tank in five different concentrations. Findings: The mortality rate was measured after 24 hours of exposure. Fish died at 250µg/ml and 100µg/ml. In histopathology analysis, hemorrhages in the inner lamellar space, moderate lamellar epithelial cell necrosis, and sloughing of the lamellar epithelium were observed in the gill. Cerebral congestion, multifocal vacuolar degeneration, and infiltration of mononuclear cells were observed in the brain. Periportal necrosis and hypertrophy of hepatocytes were observed in the liver. Renal hemorrhage, multifocal degeneration of tubular epithelium, and sinusoid expansion were observed in the kidney. Sloughing of epithelial villi, villous atrophy, cellular infiltration, and focal necrosis of epithelial cells was observed in the kidney. Novelty: This is the first report from this particular study site. Even though Oleic acid has some impact on the fish, the optimum level of oleic acid from this particular seaweed can be used as a natural medicine for human society.

Keywords: Oleic Acid, Caulerpa Scalpelliformis, Oreochromis Niloticus, Hepatotoxicity, Histopathology Analysis


  1. Pandey AK, Chauhan OP, Semwal AD. Seaweeds-a potential source for functional foods. Defence Life Science Journal. 2020;5(4):315–337. Available from: https://doi.org/10.14429/dlsj.5.15632
  2. Gephart JA, Golden CD, Asche F, Belton B, Brugere C, Froehlich HE, et al. Scenarios for Global Aquaculture and Its Role in Human Nutrition. Reviews in Fisheries Science & Aquaculture. 2021;29(1):122–138. Available from: https://doi.org/10.1080/23308249.2020.1782342
  3. El-Sappah AH, Seif MM, Abdel-Kader HH, Soaud SA, Elhamid MAA, Abdelghaffar AM, et al. Genotoxicity and Trace Elements Contents Analysis in Nile Tilapia (Oreochromis niloticus) Indicated the Levels of Aquatic Contamination at Three Egyptian Areas. Frontiers in Veterinary Science. 2022;9:818866. Available from: https://doi.org/10.3389/fvets.2022.818866
  4. Iqbal S, Atique U, Mahboob S, Haider MS, Iqbal HS, Al-Ghanim KA, et al. Effect of supplemental selenium in fish feed boosts growth and gut enzyme activity in juvenile tilapia (Oreochromis niloticus) Journal of King Saud University - Science. 2020;32(5):2610–2616. Available from: https://doi.org/10.1016/j.jksus.2020.05.001
  5. Piccinin E, Cariello M, Santis SD, Ducheix S, Sabbà C, Ntambi JM, et al. Role of Oleic Acid in the Gut-Liver Axis: From Diet to the Regulation of Its Synthesis via Stearoyl-CoA Desaturase 1 (SCD1) Nutrients. 2019;11(10):2283. Available from: https://doi.org/10.3390/nu11102283
  6. Chen D. Histological Staining and its Methods. Journal of Interdisciplinary Histopathology. 2022;10(9):1–2. Available from: https://www.ejmjih.com/ejmjih-articles/histological-staining-and-its-methods-92034.html
  7. Rocha CP, Pacheco D, Cotas J, Marques JC, Pereira L, Gonçalves AMM. Seaweeds as Valuable Sources of Essential Fatty Acids for Human Nutrition. International Journal of Environmental Research and Public Health. 2021;18(9):4968. Available from: https://doi.org/10.3390/ijerph18094968
  8. Arrese M, Barrera F, Triantafilo N, Arab JP. Concurrent nonalcoholic fatty liver disease and type 2 diabetes: diagnostic and therapeutic considerations. Expert Review of Gastroenterology & Hepatology. 2019;13(9):849–866. Available from: https://doi.org/10.1080/17474124.2019.1649981
  9. Younossi Z, Tacke F, Arrese M, Sharma BC, Mostafa I, Bugianesi E, et al. Global Perspectives on Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis. Hepatology. 2019;69(6):2672–2682. Available from: https://doi.org/10.1002/hep.30251
  10. Mahboob S, Al-Ghanim KA, Al-Balawi HF, Al-Misned F, Ahmed Z. Toxicological effects of heavy metals on histological alterations in various organs in Nile tilapia (Oreochromis niloticus) from freshwater reservoir. Journal of King Saud University - Science. 2020;32(1):970–973. Available from: https://doi.org/10.1016/j.jksus.2019.07.004
  11. Oliveira EAD, Merigueti YFFB, Ferreira IB, Garcia IS, Pereira AS, Santos RDS, et al. The Role of Nile Tilapia (Oreochromis niloticus) in the Life Cycle of Toxocara spp. Frontiers in Veterinary Science. 2021;8:685911. Available from: https://doi.org/10.3389/fvets.2021.685911
  12. Abdel-Warith AWAW, Younis EM, Al-Asgah NA, Gewaily MS, El-Tonoby SM, Dawood MAO. Role of Fucoidan on the Growth Behavior and Blood Metabolites and Toxic Effects of Atrazine in Nile Tilapia Oreochromis niloticus (Linnaeus, 1758) Animals. 2021;11(5):1448. Available from: https://doi.org/10.3390/ani11051448
  13. Hedayatirad M, Mirvaghefi A, Nematollahi MA, Forsatkar MN, Brown C. Transgenerational disrupting impacts of atrazine in zebrafish: Beneficial effects of dietary spirulina. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2020;230:108685. Available from: https://doi.org/10.1016/j.cbpc.2019.108685
  14. Yang C, Lim W, Song G. Mediation of oxidative stress toxicity induced by pyrethroid pesticides in fish. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2020;234:108758. Available from: https://doi.org/10.1016/j.cbpc.2020.108758
  15. Jawdhari A, Mihăilescu DF, Stan MS, Bălănescu MVV, Vlăsceanu RII, Staicu CA, et al. Ingestion of Polyvinylchloride Powder Particles Induces Oxidative Stress and Hepatic Histopathological Changes in Oreochromis niloticus (Nile Tilapia)—A Preliminary Study. Sustainability. 2023;15(8):6494. Available from: https://doi.org/10.3390/su15086494
  16. Farag MR, Zheng C, Gharib HSA, El-Hady E, Mahdy EAA, Abo-Elmaaty AMA, et al. Physiological and Neurobehavioral Disturbances Induced by Al2O3 Nanoparticle Intoxication in Nile Tilapia Fish: Benefits of Dietary Chamomile Essential Oil. Aquaculture Nutrition. 2023;2023:1–14. Available from: https://doi.org/10.1155/2023/6700708
  17. Cui Q, Qiu L, Yang X, Shang S, Yang B, Chen M, et al. Transcriptome profiling of the low-salinity stress responses in the gills of the juvenile Pseudopleuronectes yokohamae. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. 2019;32:100612. Available from: https://doi.org/10.1016/j.cbd.2019.100612
  18. Dawood MAO, Abdel-Razik NI, Gewaily MS, Sewilam H, Paray BA, Soliman AA, et al. β-Glucan improved the immunity, hepato-renal, and histopathology disorders induced by chlorpyrifos in Nile tilapia. Aquaculture Reports. 2020;18:100549. Available from: https://doi.org/10.1016/j.aqrep.2020.100549


© 2023 Sherlina et al.  This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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