Indian Journal of Science and Technology
Year: 2016, Volume: 9, Issue: 45, Pages: 1-6
Senthilkumar P1*, Dawn SS2 , Antony V Samrot3 , Narendra Kumar G3 and Aashrit Raj D1
1Department of Chemical Engineering, School of Bio and Chemical Engineering, Sathyabama University. [email protected] 2Centre for Waste Management, Sathyabama University. 3 Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama University.
*Author for correspondence
Senthilkumar P Department of Chemical Engineering, School of Bio and Chemical Engineering, Sathyabama University. [email protected]
Objectives: To produce, optimize and characterize poly[R]hydroxyalkanoate (PHA) from Enterobacter sp SU16. Methods: isolation of Enterobacter sp SU16 from dairy waste. Optimization of PHA production was done. Extraction of PHA was done by non-solvent precipitation method. Presence of PHA was confirmed by UV Spectrometry and TLC. Characterization of PHA was done by FTIR and GCMS analysis. Findings: Optimal PHA production was observed at 48h incubation at 35°C in 3% glucose containing medium at pH 8. PHA was found to with 3-hydroxydodecanoic acid monomers. Novelty: Enterobacter sp SU16 to accumulate polyhydroxydecanoate (PHD) monomers.
Keywords: FTIR analysis, GC-MS analysis, Enterobacter sp SU16, poly[R]hydroxyalkanoate - PHA
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