• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2016, Volume: 9, Issue: 45, Pages: 1-6

Original Article

Production, Optimization and Characterization of Poly[R]Hydroxyalkanoate from Enterobacter sp SU16


Objectives: To produce, optimize and characterize poly[R]hydroxyalkanoate (PHA) from Enterobacter sp SU16. Methods: isolation of Enterobacter sp SU16 from dairy waste. Optimization of PHA production was done. Extraction of PHA was done by non-solvent precipitation method. Presence of PHA was confirmed by UV Spectrometry and TLC. Characterization of PHA was done by FTIR and GCMS analysis. Findings: Optimal PHA production was observed at 48h incubation at 35°C in 3% glucose containing medium at pH 8. PHA was found to with 3-hydroxydodecanoic acid monomers. Novelty: Enterobacter sp SU16 to accumulate polyhydroxydecanoate (PHD) monomers. 

Keywords: FTIR analysis, GC-MS analysis, Enterobacter sp SU16, poly[R]hydroxyalkanoate - PHA


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