Regeneration of Neural Crest Cells from Human Postnatal Multipotent Dental Pulp Stem Cells

Nikhat Fatima; Sandeep Kumar Vishwakarma  and Aleem Ahmed Khan    nbsp

doi:10.17485/ijst/2017/v10i29/116353

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Regeneration of Neural Crest Cells from Human Postnatal Multipotent Dental Pulp Stem Cells

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2017/v10i29/116353

Year: 2017, Volume: 10, Issue: 29, Pages: 1-12

Original Article

Regeneration of Neural Crest Cells from Human Postnatal Multipotent Dental Pulp Stem Cells

Nikhat Fatima^1,2, Sandeep Kumar Vishwakarma² and Aleem Ahmed Khan^{2 *}

¹Pacific University, Udaipur – 313003, Rajasthan, India; [email protected] ²Central Laboratory for Stem Cell Research and Translational Medicine, CLRD, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad – 500058, Telangana, India; Sa[email protected], [email protected]

*Author For Correspondence
Aleem Ahmed Khan Central Laboratory for Stem Cell Research and Translational Medicine, CLRD, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad – 500058, Telangana, India; [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objective: To identify discarded dental tissues as source for stem cells isolation and their differentiation into neurogenic lineage for regenerative applications. Methods/Analysis: Dental pulp tissues (Both impacted and erupted) were collected from 18-40 years old individuals having proper orthodontic reasons. The long-term in vitro culture of dental pulp stem cells (DPSCs) was established in controlled condition. Population doubling analysis was performed at different passages. DPSCs were further triggered into neurogenic cells under serum free retinoic acid defined supplements. Neurospheres development and neurogenic lineage differentiation was identified. Findings: More than 80% DPSCs were found to express putative dental stem cell marker CD90. We observed all basic biological characteristics of DPSCs during long-term cultivation up to 20 passages. The diameter distribution of cultured DPSCs was quite stable and was found to be 12-18μm in diameter. Highest numbers of neuropsheres were found to be at day 14 with ~100µm in size. Expression of β tubulin-III revealed highest differentiation (>15%) towards neuronal cells. Novelty/Improvement: The present study demonstrates that DPSCs could be a potential option to make repository of neurogenic cells for regenerative applications.

Keywords: Neural Crest Cells, Neural Lineage Cells, Neurospheres, DPSCs, PDT