Indian Journal of Science and Technology
Year: 2011, Volume: 4, Issue: 1, Pages: 52-63
M. S. Dake*, M. B. Khetmalas and S. V. Amarapurkar
Dr. D. Y. Patil Biotechnology & Bioinformatics Institute, Survey No. 87/88, Mumbai–Pune Bypass Express Highway, Tathawade, Pune-411033, Maharashtra, India. [email protected]
Saccharomyces italicus has two pools of glycogen–one is the soluble pool and the remainder water insoluble linked with cell wall or β-glucans. Soluble pool of glycogen present is assigned to produce energy required for metabolic activities but the insoluble pool in the cell wall plays a special physiological role in providing the osmotolerance under ethanol stress conditions. S. italicus grown in the presence of 2-8% v/v ethanol exhibits exponential increase in insoluble glycogen content as a protective measure against the stress created across the plasma membrane by the action of ethanol. But ethanol concentration above 8%v/v reduces hydrophobic interactions thereby decreasing the insoluble glycogen content with increase in membrane permeability.
Keywords: Saccharomyces, glycogen, osmotolerance, ethanol, yeast cell wall.
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