Sensor based on polyvinyl chloride immobilized Pseudomonas striata cells as metal-ionophore.

Minakshi Datta; Susheel Mittal    and Dinesh Goyal

doi:10.17485/ijst/2009/v2i7.3

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Sensor based on polyvinyl chloride immobilized Pseudomonas striata cells as metal-ionophore.

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2009/v2i7.3

Year: 2009, Volume: 2, Issue: 7, Pages: 46-50

Original Article

Sensor based on polyvinyl chloride immobilized Pseudomonas striata cells as metal-ionophore.

Minakshi Datta, Susheel Mittal¹ and Dinesh Goyal

Dept. of Biotech. & Environmental Sci.; 1School of Chemistry & Biochem., Thapar Univ., Patiala-147004. Punjab, India.

*Author for the correspondence:
Dinesh Goyal
Dept. of Biotech. & Environmental Sci Thapar Univ.,
Patiala-147004. Punjab, India.
E-mail: [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

PVC membrane containing lyophilized cell mass of Pseudomonas striata was prepared using dibutylpthalate as the plasticizer. Anionic interferences were excluded by use of potassium salt of tetrakis (4-chlorophenyl) borate (KTCIPB). The electrode was found to be fairly selective and sensitive for the zinc ions. It exhibited a linearity range of 10-1 to 10-5 M with near nernstian slope of 26.2 mV per decade. The membrane electrode showed a sharp response time of 6-10 sec and detection limit of 5×10-5 M at 25±1ºC in the pH optima of 3-5. The interference was found to arise only from few transition metals such as Hg+2, Ag+ and Pb+2.

Keywords: Zn-metalloenzymes, Heavy metal determination, Potentiometric biosensor, Ionophore