• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2009, Volume: 2, Issue: 7, Pages: 46-50

Original Article

Sensor based on polyvinyl chloride immobilized Pseudomonas striata cells as metal-ionophore.


PVC membrane containing lyophilized cell mass of Pseudomonas striata was prepared using dibutylpthalate as the plasticizer. Anionic interferences were excluded by use of potassium salt of tetrakis (4-chlorophenyl) borate (KTCIPB). The electrode was found to be fairly selective and sensitive for the zinc ions. It exhibited a linearity range of 10-1 to 10-5 M with near nernstian slope of 26.2 mV per decade. The membrane electrode showed a sharp response time of 6-10 sec and detection limit of 5×10-5 M at 25±1ºC in the pH optima of 3-5. The interference was found to arise only from few transition metals such as Hg+2, Ag+ and Pb+2.

Keywords: Zn-metalloenzymes, Heavy metal determination, Potentiometric biosensor, Ionophore


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