Indian Journal of Science and Technology
Year: 2009, Volume: 2, Issue: 7, Pages: 46-50
Minakshi Datta, Susheel Mittal1 and Dinesh Goyal
Dept. of Biotech. & Environmental Sci.; 1School of Chemistry & Biochem., Thapar Univ., Patiala-147004. Punjab, India.
*Author for the correspondence:
Dept. of Biotech. & Environmental Sci Thapar Univ.,
Patiala-147004. Punjab, India.
E-mail: [email protected]
PVC membrane containing lyophilized cell mass of Pseudomonas striata was prepared using dibutylpthalate as the plasticizer. Anionic interferences were excluded by use of potassium salt of tetrakis (4-chlorophenyl) borate (KTCIPB). The electrode was found to be fairly selective and sensitive for the zinc ions. It exhibited a linearity range of 10-1 to 10-5 M with near nernstian slope of 26.2 mV per decade. The membrane electrode showed a sharp response time of 6-10 sec and detection limit of 5×10-5 M at 25±1ºC in the pH optima of 3-5. The interference was found to arise only from few transition metals such as Hg+2, Ag+ and Pb+2.
Keywords: Zn-metalloenzymes, Heavy metal determination, Potentiometric biosensor, Ionophore
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