Indian Journal of Science and Technology
DOI: 10.17485/ijst/2014/v7i11.3
Year: 2014, Volume: 7, Issue: 11, Pages: 1842–1846
Original Article
Srikanth Reddy Medipally1, 2, B. Naresh1, 3*, S. Manoj Kumar1 , Fatimah Md. Yusoff2 and Prathibha Devi1
1 Biotechnology Laboratory, Department of Botany, Osmania University, Hyderabad-500007, Telangana, India
2 Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
3 Department of Botany, Narendra PG College, Telangana University, Armoor, Nizamabad-503224, Telangana, India; beerelli.naresh@gmail.com
Jatropha curcas is a non-edible oil plant species and belongs to Euphorbiaceae family. It is gaining the importance as potential renewable feed-stock for biodiesel production. Here in the present study we developed an efficient micropropagation protocol through somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas. Murashigae-Skoog medium supplemented with 2, 4-D (0.5mg/l) and benzylaminopurine (5mg/l) resulted in the production of green embryogenic callus with clearly differentiated somatic embryos with a frequency of plant conversion rate from shoot tip and leaf explants was 51 ± 0.9 and 54 ± 0.6 respectively. The regenerated shoots were rooted on root induction medium, transplanted to pots and later transferred to field. The survival percentage of the transplanted plants was 33±0.1 for shoot tip and 24±0.7 for leaf explant. Therefore, this protocol could be useful for micropropagation of Jatropha curcas as effective feed-stock towards biodiesel production.
Keywords: Physic Nut, Micropropagation, MS Medium, Biodiesel, Euphorbiaceae
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