Indian Journal of Science and Technology
Year: 2008, Volume: 1, Issue: 4, Pages: 1-4
P. K. Praveen Kumar*1 , V. Mathivanan1 , M. Karunakaran1 , S. Renganathan2 and R. S. Sreenivasan3
1Department of Industrial Biotechnology, Bharath University, Chennai-600 073, India.
2Chemical Engineering Department, Anna University, Chennai-600 025, India.
3Department of Biotechnology, St. Peter’s Engineering College, Chennai-54, India.
*Author for the correspondence:
P. K. Praveen Kumar
Department of Industrial Biotechnology
Bharath University, Chennai-600 073, India.
The production of alkaline protease by Bacillus licheniformis (MTCC 1483) and Bacillus subtilis of indigenous isolates using cheapest sources- groundnut cake (N2 Source) and wheat bran (C source) was studied. The proteolytic activity was found maximum at 72 hr of the culture for both the bacterial types but the pH of the medium for maximal enzyme production varied as 9 for Bacillus licheniformis and as 8 for Bacillus subtilis. Thus investigations of this study reveal the possibility of employing cheapest nutrient source for microbial proteases that can be used on a commercial scale.
Keywords: pH, protease, Bacillus licheniformis, B. subtilis, groundnut cake, wheat bran.
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