Study of Antioxidant, Antimicrobial and Cytotoxic Activities of Ag-Co Bimetallic Nanoparticles Biosynthesized from Red Alga (Amphiroa sp.)

V Logeswari; S Yamini; P Pavithra; A Seethal Papitha; D Lakshmi

doi:10.17485/IJST/v17i19.861

Article

VIEWS 233
PDF 49

Indian Journal of Science and Technology

DOI: 10.17485/IJST/v17i19.861

Year: 2024, Volume: 17, Issue: 19, Pages: 2013-2023

Original Article

Study of Antioxidant, Antimicrobial and Cytotoxic Activities of Ag-Co Bimetallic Nanoparticles Biosynthesized from Red Alga (Amphiroa sp.)

V Logeswari¹, S Yamini¹, P Pavithra¹, A Seethal Papitha², D Lakshmi^3*

¹PG Student, Plant Biology and Plant Biotechnology, SDNB Vaishnav College for Women, Chromepet, Chennai, Tamil Nadu, India
²Research Scholar, Plant Biology and Plant Biotechnology, SDNB Vaishnav College for Women, Chromepet, Chennai, Tamil Nadu, India
³Associate Professor, Plant Biology and Plant Biotechnology, SDNB Vaishnav College for Women, Chromepet, Chennai, Tamil Nadu, India

*Corresponding Author
Email: [email protected]

Received Date:21 March 2024, Accepted Date:25 April 2024, Published Date:09 May 2024

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: To biosynthesize, evaluate, and investigate the green synthesis of silver-cobalt bimetallic nanoparticles using red alga Amphiroa sp. and its interactions with human pathogens in a colloidal condition. Methods: The marine red algal extract of Amphiroa sp. was used to synthesize the bimetallic nanoparticles of Ag and Co. For this 50mL of 10-3 aqueous Ag-Co prepared solutions were combined with 50mL of pure algal extract. It was characterized by UV-Vis, FTIR, XRD, and SEM. It was tested for its antioxidant, antibacterial, and cytotoxic activities. The antibacterial activity of bimetallic nanoparticles was tested on five human pathogens Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa. The MTT assay method was used for cytotoxic activity for MCF-7 Breast cancer cell lines. Findings: The green synthesized Amphiroa sp. bimetallic nanoparticles showed a UV-Vis spectrum absorption peak at 517nm. Analysis of the FTIR spectra verified the functional groups involved in the production of the Ag-Co nanoparticles. The diffraction pattern of silver-cobalt nanoparticles and the X-ray diffraction pattern of silver nanoparticles showed diffraction angles at 2θ values of 32.5°(15), 46.5°(11) which correspond to (111), (200), and (220). The particle size distribution, which ranges from 56 to 250nm, and the shape was revealed by SEM investigation to be cubic to rhomboidal, thus, they were confirmed to be nanoparticles as well as fine particles/particulate matter. Radical scavenging activity by DPPH, ABTS, and ferrous-reducing power assays were used to investigate the antioxidant potential. The antibacterial activity of bimetallic nanoparticles was tested on five human pathogens and with the zone of inhibition of 23, 18, and 20 mm for Pseudomonas aeruginosa, Escherichia coli and Bacillus subtilis, respectively. The cytotoxic effect was observed on MCF-7 cancer cell lines were evaluated by MTT assay. The IC50 value of our Ag-Co bimetallic sample was found to be 70.90µg/ml at 200µg/mL concentrations. Novelty: Biosynthesizing and investigating Ag-Co bimetallic nanoparticles using red alga and its characterization, antioxidant, antimicrobial and cytotoxic activity.

Keywords: Amphiroa sp.; Ag-Co Bimetallic nanoparticles (BNPs); antioxidant, antibacterial and cytotoxic activity

References

Chugh D, Viswamalya VS, Das B. Green synthesis of silver nanoparticles with algae and the importance of capping agents in the process. Journal of Genetic Engineering and Biotechnology. 2021;19(1):1–21. Available from: https://dx.doi.org/10.1186/s43141-021-00228-w
Riaz T, Nayyar S, Shahzadi T, Zaib M, Shahid S, Mansoor S, et al. The Biogenic Synthesis of Cobalt Monometallic and Cobalt–Zinc Bimetallic Nanoparticles Using Cymbopogan citratus L. Leaf Extract and Assessment of Their Activities as Efficient Dye Removal and Antioxidant Agents. Agronomy. 2022;12(10):1–17. Available from: https://dx.doi.org/10.3390/agronomy12102505
Sengani M, Grumezescu AM, Rajeswari VD. Recent trends and methodologies in gold nanoparticle synthesis – A prospective review on drug delivery aspect. OpenNano. 2017;2:37–46. Available from: https://dx.doi.org/10.1016/j.onano.2017.07.001
Zadeh FA, Bokov DO, Salahdin OD, Abdelbasset WK, Jawad MA, Kadhim MM, et al. Cytotoxicity evaluation of environmentally friendly synthesis Copper/Zinc bimetallic nanoparticles on MCF-7 cancer cells. Rendiconti Lincei. Scienze Fisiche e Naturali. 2022;33:441–447. Available from: https://dx.doi.org/10.1007/s12210-022-01064-x
Ameen F, Al-Homaidan AA. Improving the efficiency of vermicomposting of polluted organic food wastes by adding biochar and mangrove fungi. Chemosphere. 2022;286(Part 3). Available from: https://dx.doi.org/10.1016/j.chemosphere.2021.131945
Ameen F, Al-Homaidan AA. Treatment of heavy metal–polluted sewage sludge using biochar amendments and vermistabilization. Environmental Monitoring and Assessment. 2022;194(12). Available from: https://dx.doi.org/10.1007/s10661-022-10559-x
Desam NR, Al-Rajab AJ, Sharma M, Mylabathula MM, Gowkanapalli RR, Albratty M. Chemical constituents, in vitro antibacterial and antifungal activity of Mentha×Piperita L. (peppermint) essential oils. Journal of King Saud University - Science. 2019;31(4):528–533. Available from: https://dx.doi.org/10.1016/j.jksus.2017.07.013
Akinsiku AA, Dare EO, Ajani OO, Ayo-Ajayi J, Ademosun OT, Ajayi SO. Room temperature Phytosynthesis of Ag/Co bimetallic nanoparticles using aqueous leaf extract of <i>Canna indica</i>. In: 2nd International Conference on Science and Sustainable Development (ICSSD 2018)"The Role of Science in Novel Research and Advances in Technology", IOP Conference Series: Earth and Environmental Science. (Vol. 173, pp. 1-13) IOP Publishing. 2018.
Loza K, Heggen M, Epple M. Synthesis, Structure, Properties, and Applications of Bimetallic Nanoparticles of Noble Metals. Advanced Functional Materials. 2020;30(21):1–14. Available from: https://dx.doi.org/10.1002/adfm.201909260
Rostami-Vartooni A, Nasrollahzadeh M, Alizadeh M. Green synthesis of perlite supported silver nanoparticles using Hamamelis virginiana leaf extract and investigation of its catalytic activity for the reduction of 4-nitrophenol and Congo red. Journal of Alloys and Compounds. 2016;680:309–314. Available from: https://dx.doi.org/10.1016/j.jallcom.2016.04.008
Ahmed B, Hashmi A, Khan MS, Musarrat J. ROS mediated destruction of cell membrane, growth and biofilms of human bacterial pathogens by stable metallic AgNPs functionalized from bell pepper extract and quercetin. Advanced Powder Technology. 2018;29(7):1601–1616. Available from: https://dx.doi.org/10.1016/j.apt.2018.03.025
Akhter MS, Uddin MA, Barabutis N. P53 Regulates the Redox Status of Lung Endothelial Cells. Inflammation. 2020;43(2):686–691. Available from: https://dx.doi.org/10.1007/s10753-019-01150-7
Khan A, Rahman F, Ahad A, Alvi PA. Investigation of transport phenomenon and magnetic behavior of Fe doped In2O3. Physica B: Condensed Matter. 2020;592. Available from: https://dx.doi.org/10.1016/j.physb.2020.412282
Tafrihi M, Imran M, Tufail T, Gondal TA, Caruso G, Sharma S, et al. The Wonderful Activities of the Genus Mentha: Not Only Antioxidant Properties. Molecules. 2021;26(4):1–22. Available from: https://dx.doi.org/10.3390/molecules26041118
Govarthanan M, Jeon CH, Jeon YH, Kwon JH, Bae H, Kim W. Non-toxic nano approach for wastewater treatment using Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles. International Journal of Biological Macromolecules. 2020;162:1241–1249. Available from: https://dx.doi.org/10.1016/j.ijbiomac.2020.06.227
Imran A, Qamar HY, Qurban AL, Naeem H, Mariam RI, Saima AM, et al. Role of Molecular Biology in Cancer Treatment: A Review Article. Iranian Journal of Public Health. 2017;46(11):1475–1485. Available from: https://ijph.tums.ac.ir/index.php/ijph/article/view/11475
Jain N, Jain P, Rajput D, Patil UK. Green synthesized plant-based silver nanoparticles: therapeutic prospective for anticancer and antiviral activity. Micro and Nano Systems Letters. 2021;9(1):1–24. Available from: https://dx.doi.org/10.1186/s40486-021-00131-6
Danbature WL, Shehu Z, Yoro M. Silver-Cobalt Bimetallic Nanoparticles as a Nanotechnological Method for Control of Culex quinquefasciatus-Borne Diseases. In: Current Advances in Chemistry and Biochemistry . (Vol. 1, pp. 1-9) Book Publisher International (a part of SCIENCEDOMAIN International). 2021.
Kourti M, Skaperda Z, Tekos F, Stathopoulos P, Koutra C, Skaltsounis AL, et al. The Bioactivity of a Hydroxytyrosol-Enriched Extract Originated after Direct Hydrolysis of Olive Leaves from Greek Cultivars. Molecules. 2024;29(2):1–19. Available from: https://dx.doi.org/10.3390/molecules29020299
Alinaghi M, Mokarram P, Ahmadi M, Bozorg-Ghalati F. Biosynthesis of palladium, platinum, and their bimetallic nanoparticles using rosemary and ginseng herbal plants: evaluation of anticancer activity. Scientific Reports. 2024;14(1):1–15. Available from: https://doi.org/10.1038/s41598-024-56275-z
Heiligtag FJ, Niederberger M. The fascinating world of nanoparticle research. Materials Today. 2013;16(7-8):262–271. Available from: https://dx.doi.org/10.1016/j.mattod.2013.07.004
Baker C, Pradhan A, Pakstis L, Pochan DJ, Shah SI. Synthesis and Antibacterial Properties of Silver Nanoparticles. Journal of Nanoscience and Nanotechnology. 2005;5(2):244–249. Available from: https://dx.doi.org/10.1166/jnn.2005.034
Kathiraven T, Sundaramanickam A, Shanmugam N, Balasubramanian T. Green synthesis of silver nanoparticles using marine algae Caulerpa racemosa and their antibacterial activity against some human pathogens. Applied Nanoscience. 2015;5(4):499–504. Available from: https://dx.doi.org/10.1007/s13204-014-0341-2
Parveen KS, Lakshmi D. Biosynthesis of silver nanoparticles using red algae, Amphiroa fragilissima and its antibacterial potential against gram positive and gram negative bacteria. International Journal of Current Science. 2016;6(3):93–100. Available from: https://rjpn.org/ijcspub/viewpaperforall.php?paper=IJCSP10A1073
Mureed S, Naz S, Haider A, Raza A, Ul-Hamid A, Haider J, et al. Development of Multi-concentration Cu:Ag Bimetallic Nanoparticles as a Promising Bactericidal for Antibiotic-Resistant Bacteria as Evaluated with Molecular Docking Study. Nanoscale Research Letters. 2021;16(1):1–12. Available from: https://dx.doi.org/10.1186/s11671-021-03547-6
Wahab MSA, Sunarti AR. Influence of PVDF/Pebax TFC Casting Temperature towards CO2/N2 Gas Separation. Indian Journal of Science and Technology. 2017;10(2):1–5. Available from: https://dx.doi.org/10.17485/ijst/2017/v10i2/110384
Ali I, Pan Y, Lin Y, Jamil Y, Hu J, Gan Z, et al. Synthesis of Ag/Co nanoparticles by dual pulsed laser ablation for synergistic photothermal study. Applied Physics A. 2021;127(8). Available from: https://dx.doi.org/10.1007/s00339-021-04706-3
Kanwal Z, Raza MA, Riaz S, Manzoor S, Tayyeb A, Sajid I, et al. Synthesis and characterization of silver nanoparticle-decorated cobalt nanocomposites (Co@AgNPs) and their density-dependent antibacterial activity. Royal Society Open Science. 2019;6(5):1–15. Available from: https://dx.doi.org/10.1098/rsos.182135
Antony R, Marimuthu R, Murugavel R. Bimetallic Nanoparticles Anchored on Core–Shell Support as an Easily Recoverable and Reusable Catalytic System for Efficient Nitroarene Reduction. ACS Omega. 2019;4(5):9241–9250. Available from: https://dx.doi.org/10.1021/acsomega.9b01023
Jamil S, Khan SR, Bibi S, Jahan N, Mushtaq N, Rafaqat F, et al. Recent advances in synthesis and characterization of iron–nickel bimetallic nanoparticles and their applications as photo-catalyst and fuel additive. RSC Advances. 2023;13(42):29632–29644. Available from: https://dx.doi.org/10.1039/d3ra04293f
Sharma G, Kumar A, Sharma S, Naushad M, Dwivedi RP, ALOthman ZA, et al. Novel development of nanoparticles to bimetallic nanoparticles and their composites: A review. Journal of King Saud University - Science. 2019;31(2):257–269. Available from: https://dx.doi.org/10.1016/j.jksus.2017.06.012
Al-Radadi NS. Facile one-step green synthesis of gold nanoparticles (AuNp) using licorice root extract: Antimicrobial and anticancer study against HepG2 cell line. Arabian Journal of Chemistry. 2021;14(2):1–25. Available from: https://dx.doi.org/10.1016/j.arabjc.2020.102956
Kannabiran K, Mohankumar T, Gunaseker V. Evaluation of Antimicrobial Activity of Saponin Isolated from Solanum xanthocarpum and Centella asiatica. International Journal of Natural and Engineering Sciences. 2009;3:25–28. Available from: http://yeni.kmu.edu.tr/Makaleler/IJNES-Issue%201-79-2011.pdf
Perdikaki A, Galeou A, Pilatos G, Prombona A, Karanikolos GN. Ion-Based Metal/Graphene Antibacterial Agents Comprising Mono-Ionic and Bi-Ionic Silver and Copper Species. Langmuir. 2018;34(37):11156–11166. Available from: https://dx.doi.org/10.1021/acs.langmuir.8b01880
Pesando D, Caram B. Screening of Marine Algae from the French Mediterranean Coast for Antibacterial and Antifungal Activity. Botanica Marina. 1984;27(8):381–386. Available from: https://dx.doi.org/10.1515/botm.1984.27.8.381
Khalaf WY, Elias RS, Raheem LA. Design, synthesis and molecular docking study of coumarin pyrazoline derivatives against MCF-7 breast cancer cell line. Pharmacia. 2023;70(4):1487–1492. Available from: https://dx.doi.org/10.3897/pharmacia.70.e108670
Zadeh FA, Bokov DO, Salahdin OD, Abdelbasset WK, Jawad MA, Kadhim MM, et al. Cytotoxicity evaluation of environmentally friendly synthesis Copper/Zinc bimetallic nanoparticles on MCF-7 cancer cells. Rendiconti Lincei. Scienze Fisiche e Naturali. 2022;33(2):441–447. Available from: https://dx.doi.org/10.1007/s12210-022-01064-x

Copyright

© 2024 Logeswari et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)