Indian Journal of Science and Technology
Year: 2016, Volume: 9, Issue: 29, Pages: 1-8
Mahmoud Hossam1 , Armanious Wagih1 , Elenbawy Mona1 , Elhariri Mahmoud1 , Elhelw Rehab1 * and El-Din Taher Salah2
1 Department of Microbiology, [email protected]
2 Nanotechnology Center,
*Author for correspondence
Department of Microbiology
Objectives: The aim of this study to investigate MG in field cases of chronic respiratory disease (CRD) and its tissue distribution in positive cases. Methods: Polymerase Chain Reaction by different approaches was used in comparison to the culture method. The study included 385 birds, from which (1,155) samples of trachea, lung tissue and air sacs were collected from different farms of Layers, broilers and Breeders flocks located in seven governorates. Findings: Three detection methods of MG revealed that, the highest recovery rate of MG was achieved by PCR on 48 hours incubation PPLO broth (70.9%), followed by direct PCR on infected tissue (65.45%). The lowest recovery rate was for conventional cultivation method (17.66%) indicating that PCR is more sensitive. On the other hand tissue distribution pattern of MG showed higher percentage from trachea followed by air sacs then lung. Application: The PCR is superior to culture as it is rapid and sensitive for diagnosis of MG, which is compatible with surveillance and control programs of MG. Regarding tissue distribution pattern the trachea has a more privilege during diagnosis of MG infection.
Keywords: Egypt, Molecular Diagnosis, Mycoplasma gallisepticum, Poultry, Recovery
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