The usefulness of two-colour fluorescence in situ hybridization technique using chromosomal subset at metaphase and interphase stage in radiation biodosimetry

Kimio Tanaka; Mansyur Arif; and Nanao Kamada  nbsp

doi:10.17485/ijst/2009/v2i4.1

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The usefulness of two-colour fluorescence in situ hybridization technique using chromosomal subset at metaphase and interphase stage in radiation biodosimetry

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2009/v2i4.1

Year: 2009, Volume: 2, Issue: 4, Pages: 1-11

Original Article

The usefulness of two-colour fluorescence in situ hybridization technique using chromosomal subset at metaphase and interphase stage in radiation biodosimetry

Kimio Tanaka^１*, Mansyur Arif ^2,3 and Nanao Kamada⁴

¹Department of Radiobiology, Institute for Environmental Sciences, Hachazawa 2-121,Takahoko, Rokkasho, Kamikita, Aomori 039-3213, Japan
²Department of Cancer Cytogenetics, Research Institute for Radiation biology and Medicine, Hiroshima University,Kasumi-1-2-3, Minami-ku Hiroshima, Japan
³Department of Clinical Pathology, Medical Faculty, Hasanudin University, Makassar, Indonesia
⁴Atomic Bomb Survivors Relief Foundation, Kurakake, Asakita-ku, Hiroshima 739-1743, Japan

*Author for the correspondence:
Kimio Tanaka
Department of Radiobiology, Institute for Environmental Sciences,
Hachazawa 2-121,Takahoko, Rokkasho, Kamikita, Aomori 039-3213, Japan
E-mail: [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Peripheral bloods from healthy donors were irradiated by 60Co γ-rays and californium (252Cf) –neutron for 0.5 to 4 Gy and the frequencies of the translocations were detected by two colour fluorescence in situ hybridization (FISH). Chromosomes 1, 2 and 3 and chromosomes 6, 7 and 9 were labeled by rodamine-dUTP and fluorescein-dUTP as viewing red or green signal on metaphase chromosomes, respectively. In separate observation, translocation was detected on the interphase cells by the two color FISH method using whole chromosome painting probes of chromosomes 4 and 9. Detected translocations on the metaphase and interphase cells showed clear dose response relationships. Translocations induced by 60Co γ-rays, 252Cf neutrons, and those in atomic bomb survivors were able to detect easily by both interphase FISH and metaphase FISH using two colour of chromosome subsets. These results suggest that scoring of translocations by two colour FISH of chromosome subsets can serve as more sensitive and applicable biological dosimeter for short or long-term radiation effects.

Keywords: Interphase FISH, biodosimetry, atomic bomb, neutron, γ rays