Indian Journal of Science and Technology
Year: 2010, Volume: 3, Issue: 3, Pages: 349-354
Bhanu Revathi K1 , Kolluru V. A. Ramaiah2 , Alan G. Hinnebusch3 , Abani K. Bhuyan4 , Sivasai KSR1 , Akulapalli Sudhakar5,6,7 and Laxminarayana Burela1,*
1. Dept. of Biotechnology, Sreenidhi Instt. of Science and Technol., Yamnampet, Ghatkesar, Hyderabad, India.
2. Dept. of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
3. Laboratory of Gene Regulation and Development, National Instt. of Child Health and Human Development, National Instt. of Health, Bethesda, MD 20892, USA.
4. School of Chemistry, University of Hyderabad, Hyderabad, India.
5. Cell Signaling and Tumor Angiogenesis Lab, Dept. of Genetics, Boys Town National Research Hospital, Omaha, NE, USA.
6. Dept. of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, USA
7. Dept. of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omha, NE, USA
Cell-free translation systems have developed significantly over the last 2 decades and improvements in yield have resulted in their use for in vitro protein production in the laboratory. Such cell free systems enable the expression of cytotoxic, regulatory or unstable proteins that cannot be expressed in living cells. We compare the uses of different cell-free translation systems and review recent findings that support the possibility of scaling up and thereby enhancing the industrial application of wheat germ cell free protein expression.
Keywords: Cell free biology, in vitro protein expression, scale up of translation, wheat germ extract
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