Indian Journal of Science and Technology

Abstract

This study provides scope for spectroscopic method that can be used as in-vitro test to assess the potent of vaccination instead of conventional in-vivo test. India tops in cattle wealth as well as in milk production in the world. Livestock are to be regularly vaccinated to protect them from many infectious and contagious diseases. We investigated the changes in protein and lipids levels in the pre and post vaccinated sera samples of bovine using FTIR spectral analysis. Two groups of seven animals were vaccinated with Haemorrhagic Septicaemia Vaccine (HSV) and Black Quarter Vaccine (BQV). Pre and post vaccinated sera samples of cattle were collected and tested using Fourier Transform Infra Red (FTIR) spectrometer. Spectroscopic method of blood analysis is an alternate technique to the clinical method since they require fewer samples and provide more information. The variation observed in peaks indicating the change in protein and lipids levels in the animals was due to introduce of antigens. Compared to ELISA, spectral analysis is cost effective test besides it requires small amount of sample for analysis and conforms to bioethics. In vivo challenge test can be replaced once this procedure is standardized which can satisfy the CPCSEA- “Committee for the Purpose of Control and Supervision on Experiments on Animal”- which imposes stringent regulations to use animal for experiments.
Keywords: Cattle, Livestock, Vaccination, spectroscopy, FTIR spectrometer.