Indian Journal of Science and Technology
Year: 2015, Volume: 8, Issue: 23, Pages: 1-5
K. Sabeena Beevi* and G. R. Bindu
Department of Electrical Engineering, College of Engineering Trivandrum, Thiruvananthapuram - 695016, Kerala, India;
[email protected], [email protected]
This paper proposes a unique detection technique to identify nuclei from microscopy images of breast histopathology slides. The method involves stain normalization, color decomposition and hysteresis thresholding. A comparative analysis of different pre-processed images on nuclei detection reveals an accuracy of 97% for Blue Ratio (BR) images. The technique uses three color channels for accurate detection of nuclei. An analysis of two stain normalization procedure is also presented.
Keywords: Blue Ratio Image, Histopathology, Stain Normalization
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