• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2023, Volume: 16, Issue: 21, Pages: 1555-1562

Original Article

Aniline Formaldehyde Cross-Linked Polyaniline Magnetic Nanocomposite with Core-Shell Structure As A New Platform for Enzyme Immobilization

Received Date:04 October 2022, Accepted Date:10 May 2023, Published Date:31 May 2023


Objectives: The industrial application of an enzyme is governed by its thermodynamic properties and stability, which can be effectively improved by immobilizing enzymes onto suitable supports. The present work is done by immobilizing a-amylase onto aniline formaldehyde condensate cross-linked polyaniline magnetic nanocomposite. Methods: Physiochemical characterization of synthesized Fe3O4-CLPANI nanocomposite was done by FT-IR, XRD, TG, and SEM images. Biochemical characterization related to immobilization efficiency, the kinetic parameters Vmax and the Km value, thermal stability, storage stability and reusability of immobilized enzymes were determined. Findings: Both physiochemical and biochemical characterization led to the conclusion that Fe3O4–CLPANI immobilized amylase indicated better thermal stability than that of free enzyme and it tailored to the requirement as an industrial catalyst. Novelty: To the best of our knowledge, no literature is available on the immobilization of the enzyme to Fe3O4–CLPANI composites with core-shell structure.

Keywords: Nanocomposite; Amylase; Immobilization; Thermal stability; Thermal deactivation a


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© 2023 Augustine et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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