• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2018, Volume: 11, Issue: 41, Pages: 1-7

Original Article

Biosorption of Mercury and Nickel in Vitro by Microalga Chlorella sp. in Solution and Immobilized in Dry Fruit of Squash (Luffa Cylindrica)


Objectives: This work aimed to determine the biosorption capacity of Mercury and Nickel by the microalga Chlorella sp. present in solution and immobilized in dried fruit of Luffa cylindrica. Methods/Statistical Analysis: The algal biomass was bioaugmented in photobioreactors with 4mM Agrimins for 18±1 days in constant agitation. For the immobilization of the microalga, the sponge was used as support. The mercury and nickel removal capacity was determined by making daily growth measurements at 647 nm with a UV-vis SpectroquantPharo 300 Merck spectrophotometer. With the results an ANOVA and the Tukey test (p-value≤0.05) were performed in the InfoStat software. Findings: The results obtained showed that the microalga adheres to the fibers of the scourer, with an average immobilization of 1.58g of microalga/scourer fragment of 2.5x2.8Cm at 18 days. The results showed significant statistical differences (p-value<0.05) between, microalga in solution, immobilized, Hg and Ni concentration and between the interactions of these factors. Phycoremediation is an efficient removal technique of Hg and Ni present in contaminated water, and is potentialized when algal cells are attached to substrates that provide protection. Improvements/Applications: immobilized microalgae are an efficient alternative to remove environmental contaminants, but the type of substrate that contains the biosorbent is a key factor in the success of this biotechnology.

Keywords: Chlorella, Bioremediation, Biosorption, Mercury, Nickel


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