Indian Journal of Science and Technology
Year: 2019, Volume: 12, Issue: 21, Pages: 1-8
Mohd Aris Amira1*, Tengku Zainal Elida1, Abdul Hazid Umi Marshida1, M. F. Z. R. Yahya1 and Affouad Saba2
1Center of Pure and Applied Biology, Department of Biomolecular Sciences, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor, Malaysia; [email protected], [email protected], [email protected], [email protected]
2Faculty of Dentistry, Universiti Teknologi MARA (UiTM), 47000 Sungai Buloh, Selangor, Malaysia; [email protected]
*Author for correspondence
Mohd Aris Amira
Center of Pure and Applied Biology, Department of Biomolecular Sciences, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor, Malaysia. Email: [email protected]
Objectives: The study was to examine the effect of rhamnolipid biosurfactant on Streptococcus mutans biofilm formation on orthodontic appliances. Methods/Statistical Analysis: Several properties of S. mutans cells such as formation of biofilm, detachment ability, the surface properties were changed after three different concentrations of biosurfactant treatment which were 10 mg/ml, 5 mg/ml and 2.5 mg/ml. S. mutans biofilms were disrupted by rhamnolipids produced by Pseudomonas aeruginosa ATCC10145 at different concentrations and chlorhexidine digluconate 0.12% (v/v) was used as positive control. Findings: The biosurfactant showed some antimicrobial activities against S. mutans with Minimum Inhibitory Concentration (MIC) varied from 10 mg/ml to 0.01 mg/ml. MIC were observed at 1.25 mg/ml by microdilution 96-well plate method. The Minimum Bactericidal Concentration (MBC) was detected at a higher concentration of 10 mg/ml by plating onto agar. The best treatment of dental biofilm formation was determined at 10 mg/ml showing the highest percentage of biofilm detachment which was 89.53%. The biofilm development is commonly known as strongly dependent on hydrophobicity/hydrophilicity substrate properties using contact angle measurement. Higher percentage (89.53%) of biofilm detachment determined at polystyrene surface compared to stainless steel surface with the aid of rhamnolipid. Rhamnolipid displayed a significant potential as disrupting agents against established biofilms produced by several bacterial and fungal species. Application/Improvements: P. aeruginosa were successfully extracted their crude biosurfactant to treat the growth of biofilm. Future studies can be performed on specific mechanisms on how biosurfactant inhibit and disrupt biofilm growth.
Keywords: Biofilm Formation, Biosurfactant, Pseudomonas Aeruginosa, Streptococcus mutans
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