Diversity of Endophytic Bacteria from Marine Associated Plant Leaves

B Megala; P Thirumalai Vasan

doi:10.17485/IJST/v16i23.88

Article

Diversity of Endophytic Bacteria from Marine Associated Plant Leaves

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Indian Journal of Science and Technology

DOI: 10.17485/IJST/v16i23.88

Year: 2023, Volume: 16, Issue: 23, Pages: 1726-1732

Original Article

Diversity of Endophytic Bacteria from Marine Associated Plant Leaves

B Megala^1*, P Thirumalai Vasan²

¹Research scholar, PG and Research Department of Biotechnology, Srimad Andavan Arts and Science College (Autonomous), Affiliated to Bharathidasan University, T.V.Kovil, Trichy, India
²Head, PG and Research Department of Biotechnology, Srimad Andavan Arts and Science College (Autonomous), Affiliated to Bharathidasan University, T.V. Kovil, Trichy, India

*Corresponding Author
Email: [email protected]

Received Date:12 January 2023, Accepted Date:31 May 2023, Published Date:09 June 2023

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: To study endophytic bacterial population from 3 marine plants such as Avicennia marina, Salicornia brachiata and Suaeda maritima. Methods: The fresh leaves from the above plants were collected at Kattumavadi, Kottaipattinam and Sethubhavachathiram mangrove ecosystems during the period of December month. The leaves were surface sterilized and the inner tissues of the leaf bits were macerated and serially diluted for the isolation of bacterial endophytes and grown on Nutrient Agar (NA) Petri plates. The endophytic bacteria grown in the nutrient agar was identified with the help of gram staining and biochemical assessments. The statistical method of endophytic bacterial diversity was measured in phrases of Shannon’s and Simpson’s indices. Findings: Absolutely 219 colonies are recorded in three marine plant leaves consisting of Avicennia marina (109), Salicornia brachiata (52) and Suaeda maritima (58). The nine microorganisms were recognized and denoted by letter code such as BMBT 1 to BMBT 9 consisting of Bacillus amyloliquefaciens, B. Megaterium, B. Subtilis, Pseudomonas aeroginosa, Corynebacterium sp, B. licheniformis, Citrobacter sp, Halomonas sp and Mycobacterium sp. The results confirmed that most of the bacterial diversity belonged to Bacillaceae family. Endophytic bacterial range indices have been measured using a statistical method, which revealed variations in plant variety and species richness. The Simpson’s diversity indices of 3 marine leaves inclusive of Avicennia marina (0.345), Salicornia brachiata (0.368) and Suaeda maritima (0.341) have been cited with the maximum for Salicornia brachiata in comparison with different plant leaves. Novelty: These novel natural merchandise from marine Bacillus sp have been employed for the use of drugs, pesticides, carotenoids, and tools for bioremediation of heavy metallic toxicity.

Keywords: Endophytic bacteria; Avicennia marina; Salicornia brachiata; Suaeda maritima; Shannon and Simpson diversity; Bacillus subtilis

References

Mishra S, Bhattacharjee A, Sharma S. An Ecological Insight into the Multifaceted World of Plant-Endophyte Association. Critical Reviews in Plant Sciences. 2021;40(2):127–146. Available from: https://doi.org/ 10.1080/07352689.2021.1901044
Gagné-Bourque F, Mayer BF, Charron JBB, Vali H, Bertrand A, Jabaji S. Accelerated Growth Rate and Increased Drought Stress Resilience of the Model Grass Brachypodium distachyon Colonized by Bacillus subtilis B26. PLOS ONE. 2015;10(6):e0130456. Available from: https://doi.org/10.1371/journal.pone.0130456
Mgbechidinma CL, Akan OD, Zhang C, Huang M, Linus N, Zhu H, et al. Integration of green economy concepts for sustainable biosurfactant production – A review. Bioresource Technology. 2022;364:128021. Available from: https://doi.org/10.1016/j.biortech.2022.128021
Tan RX, Zou WX. Endophytes: a rich source of functional metabolites . Natural Product Reports. 2001;18(4):448–459. Available from: https://doi.org/10.1039/b100918o
Christakis CA, Daskalogiannis G, Chatzaki A, Markakis EA, Mermigka G, Sagia A, et al. Endophytic Bacterial Isolates From Halophytes Demonstrate Phytopathogen Biocontrol and Plant Growth Promotion Under High Salinity. Frontiers in Microbiology. 2021;12. Available from: https://doi.org/10.3389/fmicb.2021.681567
Eid AM, Fouda A, Abdel-Rahman MA, Salem SS, Elsaied A, Oelmüller R, et al. Harnessing Bacterial Endophytes for Promotion of Plant Growth and Biotechnological Applications: An Overview. Plants. 2021;10(5):935. Available from: https://doi.org/10.3390/plants10050935
Tam HT, Phuong TV, Diep CN. Isolation and identification of endophytic bacteria associated with Rhizophora mucronate and Avicennia alba of Nam Can district, Ca Mau Mangrove Ecosystem. International Journal of Innovations in Engineering and Technology. 2018;10:147–159. Available from: http://dx.doi.org/10.21172/ijiet.141.07
Simpson EH. Measurement of Diversity. Nature. 1949;163(4148):688. Available from: https://doi.org/10.1038/163688a0
Shannon CE, Weaver W. A Mathematical Theory of Communication. Bell System Technical Journal. 1948;27(4):623–656. Available from: https://people.math.harvard.edu/~ctm/home/text/others/shannon/entropy/entropy.pdf
Pielou EC. Ecological Diversity. New York. John Wiley and Sons. 1975.
Prakamhang J, Minamisawa K, Teamtaisong K, Boonkerd N, Teaumroong N. The communities of endophytic diazotrophic bacteria in cultivated rice (Oryza sativa L.) Applied Soil Ecology. 2009;42(2):141–149. Available from: https://doi.org/10.1016/J.APSOIL.2009.02.008
Rori C, Kandou F, Tangapo A. Isolation and Antibacterial Test of Endophytic Bacteria of Avicennia marina Mangroves. 2020. Available from: https://ojs3.unpatti.ac.id/index.php/koli/article/view/2411
Jaeyoun L, Soohyun U, Hyun KS. Metabolomic analysis of halotolerant endophytic bacterium Salinivibrio costicola isolated from Suaeda maritima (L.) dumort. Frontiers in Molecular Biosciences. 2022;(9). Available from: https://doi.org/10.3389/fmolb.2022.967945
Berg G, Krechel A, Ditz M, Sikora RA, Ulrich A, Hallmann J. Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiology Ecology. 2005;51(2):215–229. Available from: https://doi.org/10.1016/j.femsec.2004.08.006
Hardoim PR, Hardoim CCP, Overbeek LSV, Elsas JDV. Dynamics of Seed-Borne Rice Endophytes on Early Plant Growth Stages. PLoS ONE. 2012;7(2):e30438. Available from: https://doi.org/10.1371/journal.pone.0030438
Ren JHH, Li H, Wang YFF, Ye JRR, Yan AQQ, Wu XQQ. Biocontrol potential of an endophytic Bacillus pumilus JK-SX001 against poplar canker. Biological Control. 2013;67(3):421–430. Available from: https://doi.org/10.1016/j.biocontrol.2013.09.012
Saïdi S, Chebil S, Gtari M, Mhamdi R. Characterization of root-nodule bacteria isolated from Vicia faba and selection of plant growth promoting isolates. World Journal of Microbiology and Biotechnology. 2013;29(6):1099–1106. Available from: https://doi.org/10.1007/s11274-013-1278-4
Rosenblueth M, Martínez-Romero E. Bacterial Endophytes and Their Interactions with Hosts. Molecular Plant-Microbe Interactions. 2006;19(8):827–837. Available from: https://doi.org/ 10.1094/MPMI-19-0827
Raaijmakers JM, Paulitz TC, Steinberg C, Alabouvette C, Moënne-Loccoz Y. The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant and Soil. 2009;321(1-2):341–361. Available from: https://doi.org/ 10.1007/s11104-008-9568-6
Berg G, Hallmann J. Control of Plant Pathogenic Fungi with Bacterial Endophytes. In: BS, CB, aTNS., eds. Soil Biology. (Vol. 53, pp. 53-69) Springer Berlin Heidelberg. 2006.
Compant S, Clément C, Sessitsch A. Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry. 2010;42(5):669–678. Available from: https://doi.org/10.1016/j.soilbio.2009.11.024
Gasser I, Cardinale M, Müller H, Heller S, Eberl L, Lindenkamp N, et al. Analysis of the endophytic lifestyle and plant growth promotion of Burkholderia terricola ZR2-12. Plant and Soil. 2011;347(1-2):125–136. Available from: https://doi.org/10.1007/s11104-011-0833-8
Prakamhang J, Minamisawa K, Teamtaisong K, Boonkerd N, Teaumroong N. The communities of endophytic diazotrophic bacteria in cultivated rice (Oryza sativa L.) Applied Soil Ecology. 2009;42(2):141–149. Available from: https://doi.org/10.1016/J.APSOIL.2009.02.008
Alishahi F, Alikhani HA, Khoshkholgh-Sima NA, Etesami H. Mining the roots of various species of the halophyte Suaeda for halotolerant nitrogen-fixing endophytic bacteria with the potential for promoting plant growth. International Microbiology. 2020;23(3):415–427. Available from: https://doi.org/10.1007/s10123-019-00115-y
Singh S, Ghadge VA, Kumar P, Mathew DE, Dhimmar A, Sahastrabudhe H, et al. Biodiversity and antimicrobial potential of bacterial endophytes from halophyte Salicornia brachiata. Antonie van Leeuwenhoek. 2021;114(5):591–608. Available from: https://doi.org/10.1007/s10482-021-01544-4
Ni J, Li F, Li Y, Lu L, Xie Y, Liu X, et al. Diversity and antibacterial activity of endophytic bacteria isolated from Avicennia marina. Southwest China Journal of Agricultural Sciences. 2020;33(9):1991–1998. Available from: https://doi.org/10.30466/vrf.2021.529714.3174

Copyright

© 2023 Megala & Vasan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)