Indian Journal of Science and Technology

Abstract

Objective: This study evaluated in vitro the growth promotion activity of endophytic bacteria extracted from different tissues of Dichantium aristatum from livestock farms localized in the zone of Santiago de Tolú, Sucre Department, Colombia. Methods/Statistical Analysis: Endophytic bacteria from different tissues were isolated, and the population was determined in CFU/g of tissue. Each isolated morphotype was used to quantitatively and qualitatively evaluate the BNF, PS, siderophore and indole acetic acid production in vitro. The bacteria that showed this activity were sequenced for identification for sequencing of the 16S rDNA gene for eubacteria. 62 morphotypes of endophyte bacteria from different Angleton pasture tissues were isolated, of which 28 had siderophore production, five ammonium ion production, 12 soluble phosphates and six indole acetic acid in mg/L. The population of these bacteria varied, from 3.210 × 106 ± 1.2 × 108 CFU/g of tissue, with higher quantity found in the Angleton pasture roots. Findings: The results of the sequencing showed the presence of a high percentage of similarity with the bacterial species Serratia marcescens, Enterobacter cloacae and Bacillus cereus, presenting phosphate solubilization, reduction of N2 to ammonium, and production of indole acetic acid and siderophores. Application: The findings of this study constitute important alternatives to mitigate the impact of the continuous use of pesticides in the production agricultural; the use of endophytic bacteria associated with vegetable species with the ability to produce multiple benefits has gained attention. Studies have shown the goodnesses of plant growth promoting-bacteria as an alternative for obtaining biofertilizers, with the ability to stimulate the growth and productivity of plants, making it are possible to improve the yield of crops.

Keywords: Biological Nitrogen Fixation, Indole Acetic Acid, Pasture, Phosphate Solubilization, Siderophore