• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2009, Volume: 2, Issue: 1, Pages: 1-8

Original Article

Establishment of mature axillary bud culture of sugarcane and overcoming persistent culture contamination


Several methods to eradicate contaminants from sugarcane mature axillary budculture including bud location/age, surface sterilization and thermal and chemical treatments of buds are examined. A spectacular improvement in the decontamination rate to 95.8 % was obtained when the heat treatment 61 °C for 30 min. was initially applied, followed by either 52 or 57 °C for 30 min, 24 hours apart. The various methods investigated and the successful rates of decontamination of sugarcane axillary buds may have a wider application in other plant tissue culture systems with known persistent contamination.

Keywords: axillary bud culture, tissue culture contamination, disease free stock, sugarcane 


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