Indian Journal of Science and Technology
Year: 2009, Volume: 2, Issue: 1, Pages: 1-8
M.E. WagihA , S.W. AdkinsB and K. AttiaC
ADepartment of Biology, Faculty of Science, University of New Brunswick, Saint John, NB, Canada.
BDepartment of Agriculture, The University of Queensland, Brisbane, Qld 4072
CInstitute of Genetic Engineering, Fudan University, Shanghai, 200433, China
*Author for the correspondence:
Department of Biology, Faculty of Science,
University of New Brunswick, Saint John, NB, Canada
E-mail: [email protected]
Several methods to eradicate contaminants from sugarcane mature axillary budculture including bud location/age, surface sterilization and thermal and chemical treatments of buds are examined. A spectacular improvement in the decontamination rate to 95.8 % was obtained when the heat treatment 61 °C for 30 min. was initially applied, followed by either 52 or 57 °C for 30 min, 24 hours apart. The various methods investigated and the successful rates of decontamination of sugarcane axillary buds may have a wider application in other plant tissue culture systems with known persistent contamination.
Keywords: axillary bud culture, tissue culture contamination, disease free stock, sugarcane
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