• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2023, Volume: 16, Issue: 24, Pages: 1777-1786

Original Article

Evaluation of Antioxidant and Antibacterial Property of Microwave Assisted Green Synthesis of Fe3O4 -MgO Nanocomposites

Received Date:18 March 2023, Accepted Date:01 June 2023, Published Date:19 June 2023


Objectives: To synthesize Magnesium oxide, iron oxide nanoparticles and Fe3O4-MgO nanocomposite using agro waste of lemon peel extract (LPE) and to examine their antioxidant and antibacterial activity. Methods: MgO, Fe3O4 NPs and Fe3O4–MgO nanocomposite by microwave-assisted technique using LPE. Feature techniques such as UV-Visible spectroscopy, X-ray diffraction (XRD), Thermo gravimetric analysis (TGA) and Scanning electron microscope (SEM) were performed to characterize the prepared material in terms of morphology and structure. Furthermore, Fourier transform infrared (FTIR) spectroscopy analysis was used to identify the possible functional groups present in the synthesis of MgO, Fe3O4 NPs and Fe3O4-MgO nanocomposite. Finding: Powder XRD confirmed the formation of MgO, Fe3O4 NPs and Fe3O4- MgO nanocomposite, SEM analysis confirmed that Iron oxide and Magnesium oxide nanoparticles and Fe3O4-MgO nanocomposite are mostly in spherical shape, while TG analysis indicated the greater thermal stability of nanoparticles as well as nanocomposite. All Synthesized nanoparticles and nanocomposite showed good antibacterial activity against gram positive and gram-negative bacterial strains (IC50 value are 8.32, 8.15 & 8.13 and 7.23, 7.57 & 7.47 mg/mL respectively). These metal oxide NPs showed antioxidant activities against DPPH (less than 85%) but enhancing activity was found for Fe3O4- MgO nanocomposite (89.95%). Novelty: These results suggested that LPE derived Fe3O4-MgO nanocomposites can be used as an effective antioxidant and antibacterial agent in biomedical applications.

Keywords: Lemon Peels Extract; Green Synthesis; Nanocomposites; Antioxidant Activity; Hemolytic Assay; Antibacterial Activity


  1. Nabi G, Qurat-Ul-Aain, Khalid NR, Tahir MB, Rafique M, Rizwan M, et al. A Review on Novel Eco-Friendly Green Approach to Synthesis TiO2 Nanoparticles Using Different Extracts. Journal of Inorganic and Organometallic Polymers and Materials. 2018;28(4):1552–1564. Available from: https://doi.org/10.1007/s10904-018-0812-0
  2. Nabi G, Ain QUU, Tahir MB, Riaz KN, Iqbal T, Rafique M, et al. Green synthesis of TiO2 nanoparticles using lemon peel extract: their optical and photocatalytic properties. International Journal of Environmental Analytical Chemistry. 2022;102(2):434–442. Available from: https://doi.org/10.1080/03067319.2020.1722816
  3. Balaba N, Jaerger S, Horsth DFL, Primo JDO, Correa JDS, Bittencourt C, et al. Polysaccharides as Green Fuels for the Synthesis of MgO: Characterization and Evaluation of Antimicrobial Activities. Molecules. 2023;28(1):142. Available from: https://doi.org/10.3390/molecules28010142
  4. Saikia JP, Paul S, Konwar BK, Samdarshi SK. Nickel oxide nanoparticles: A novel antioxidant. Colloids and Surfaces B: Biointerfaces. 2010;78(1):146–148. Available from: https://doi.org/10.1016/j.colsurfb.2010.02.016
  5. Banerjee S, Saikia JP, Kumar A, Konwar BK. Antioxidant activity and haemolysis prevention efficiency of polyaniline nanofibers. Nanotechnology. 2010;21(4):045101. Available from: https://doi.org/10.1088/0957-4484/21/4/045101
  6. Anjana VN, Joseph M, Francis S, Joseph A, Koshy EP, Mathew B. Microwave assisted green synthesis of silver nanoparticles for optical, catalytic, biological and electrochemical applications. Artificial Cells, Nanomedicine, and Biotechnology. 2021;49(1):438–449. Available from: https://doi.org/10.1080/21691401.2021.1925678
  7. Lagashetty A, Ganiger SK, K. PR, Reddy S, Pari M. Microwave-assisted green synthesis, characterization and adsorption studies on metal oxide nanoparticles synthesized using Ficus Benghalensis plant leaf extracts. New Journal of Chemistry. 2020;44(33):14095–14102. Available from: https://doi.org/10.1039/D0NJ01759K
  8. Panchal P, Paul DR, Sharma A, Hooda D, Yadav R, Meena P, et al. Phytoextract mediated ZnO/MgO nanocomposites for photocatalytic and antibacterial activities. Journal of Photochemistry and Photobiology A: Chemistry. 2019;385:112049. Available from: https://doi.org/10.1016/j.jphotochem.2019.112049
  9. Roy D, Pal A, Pal T. Electrochemical aspects of coinage metal nanoparticles for catalysis and spectroscopy. RSC Advances. 2022;12(19):12116–12135. Available from: https://doi.org/10.1039/D2RA00403H
  10. Pugazhendhi A, Prabhu R, Muruganantham K, Shanmuganathan R, Natarajan S. Anticancer, antimicrobial and photocatalytic activities of green synthesized magnesium oxide nanoparticles (MgONPs) using aqueous extract of Sargassum wightii. Journal of Photochemistry and Photobiology B: Biology. 2019;190:86–97. Available from: https://doi.org/10.1016/j.jphotobiol.2018.11.014
  11. Ramesh R, Geerthana M, Prabhu S, Sohila S. Synthesis and Characterization of the Superparamagnetic Fe3O4/Ag Nanocomposites. Journal of Cluster Science. 2017;28(3):963–969. Available from: https://doi.org/10.1007/s10876-016-1093-9
  12. Kurnaz YN, Kurşun BF, Koç MM, Nartop D. Characterization of magnetic Fe3O4@ SiO2 nanoparticles with fluorescent properties for potential multipurpose imaging and theranostic applications. Journal of Materials Science: Materials in Electronics. 2020;31(20):18278. Available from: https://doi.org/10.1007/s10854-020-04375-7
  13. Chowdhury AH, Ghosh S, Islam SM. Flower-like AgNPs@m-MgO as an excellent catalyst for CO2 fixation and acylation reactions under ambient conditions. New Journal of Chemistry. 2018;42(17):14194–14202. Available from: https://doi.org/10.1039/C8NJ02286K
  14. Meresht AS, Ezzatzadeh E, Dehbandi B, Salimifard M, Rostamian R. Fe3O4 CuO Nanocomposite Promoted Green Synthesis of Functionalized Quinazolines Using Water Extract ofLettuce Leavesas Green Media: Study of Antioxidant Activity. Polycyclic Aromatic Compounds. 2022;42(7):4793–4808. Available from: https://doi.org/10.1080/10406638.2021.1913426
  15. Zare EN, Lakouraj MM, Baghayeri M. Electro-Magnetic Polyfuran/Fe3O4 Nanocomposite: Synthesis, Characterization, Antioxidant Activity, and Its Application as a Biosensor. International Journal of Polymeric Materials and Polymeric Biomaterials. 2015;64(4):175–183. Available from: https://doi.org/10.1080/00914037.2014.936588
  16. Gulcin I. Antioxidants and antioxidant methods: an updated overview. Archives of Toxicology. 2020;94(3):651–715. Available from: https://doi.org/10.1007/s00204-020-02689-3
  17. Khan AU, Khan QU, Tahir K, Ullah S, Arooj A, Li B, et al. A Tagetes minuta based eco-benign synthesis of multifunctional Au/MgO nanocomposite with enhanced photocatalytic, antibacterial and DPPH scavenging activities. Materials Science and Engineering: C. 2021;126:112146. Available from: https://doi.org/10.1016/j.msec.2021.112146
  18. Mishra A, Pradhan D, Halder J, Biswasroy P, Rai VK, Dubey D, et al. Metal nanoparticles against multi-drug-resistance bacteria. Journal of Inorganic Biochemistry. 2022;237:111938. Available from: https://doi.org/10.1016/j.jinorgbio.2022.111938


© 2023 Anigol et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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