Genetic analysis of low-density polyethylene degrading bacteria from plastic dump sites

P Jayashree Lakshmi; K Vanmathi Selvi

doi:10.17485/IJST/v13i48.2066

Article

Genetic analysis of low-density polyethylene degrading bacteria from plastic dump sites

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Indian Journal of Science and Technology

DOI: 10.17485/IJST/v13i48.2066

Year: 2020, Volume: 13, Issue: 48, Pages: 4732-4738

Original Article

Genetic analysis of low-density polyethylene degrading bacteria from plastic dump sites

P Jayashree Lakshmi^1*, K Vanmathi Selvi¹

¹Department of Microbiology, Sri Akilandeswari Women’s College, Vandavasi, T.V.Malai, Tamil Nadu, India

*Corresponding Author
Email: [email protected]

Received Date:16 November 2020, Accepted Date:22 December 2020, Published Date:04 January 2020

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objective: The principle goal of this examination is to screen bacteria having the ability to debase Low density polyethylene. Bacteria were disengaged from different plastic dump locales. Methods: The secludes acquired were screened for their capacity to use polyethylene as sole carbon source in the mineral salt medium (MSM). Among the isolated strains, Pseudomonas putida and Pseudomonas fluorescens could able to utilize Low Density Polyethylene (LDPE) as carbon source. Further to the preliminary screening, gene specific primers for alkane monooxygenase was synthesized and gradient PCR was performed to determine the presence of alkane monooxygenase gene in the bacterial isolates. Results: Among the four bacteria selected, three bacteria such as Pseudomonas putida and Pseudomonas fluorescens could able to express ALKB gene. Hence, those two bacteria may produce the key enzyme alkane monoxygenase, which is a crucial enzyme for the biotransformation of many xenobiotic compounds including LDPE. Conclusion: From this investigation it is inferred that organisms local to soil can possibly degradeplastic with proper method of time.

Keywords: Low density polyethylene; Biodegradation; Bacteria; alkane monoxygenase; invitro Biodegradation assay

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Copyright

© 2020 Jayashree Lakshmi & Vanmathi Selvi.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)