Hepatoprotective Activity of Merremia tridentata against Carbon Tetrachloride Induced Hepatotoxicity in Rats

M Manimegalai; S Sanjay Prasad; R Visvanand; T S Subha; L Suguna

doi:10.17485/IJST/v15i45.1232

Article

Hepatoprotective Activity of Merremia tridentata against Carbon Tetrachloride Induced Hepatotoxicity in Rats

VIEWS 681
PDF 176

Indian Journal of Science and Technology

DOI: 10.17485/IJST/v15i45.1232

Year: 2022, Volume: 15, Issue: 45, Pages: 2482-2491

Original Article

Hepatoprotective Activity of Merremia tridentata against Carbon Tetrachloride Induced Hepatotoxicity in Rats

M Manimegalai^1,2, S Sanjay Prasad³, R Visvanand⁴, T S Subha^1*, L Suguna⁵

¹Department of Botany, Bharathi Women’s college, Chennai, Tamilnadu, India
²Department of Biotechnology, Dr.M.G.R.Educational and Research Institute, University, Chennai, 95, Tamilnadu, India
³Department of Microbiology, CMS College of Science and Commerce, India
⁴Department of Biotechnology, Rathnavel Subramaniyam College of Arts and Science, Coimbatore, Tamilnadu, India
⁵CSIR-Central Leather Research Institute, Chennai, Tamilnadu, India

*Corresponding Author
Email: [email protected]

Received Date:11 June 2022, Accepted Date:22 September 2022, Published Date:07 December 2022

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: Liver performs a variety of function including storage and purification of blood, secretion of bile, synthesis of plasma proteins and drug detoxification. In an attempt to study the biochemical and connective tissue metabolism during the progression of hepatic fibrosis and to gain more insight into the mechanisms leading to the altered metabolic processes in human hepatic fibrosis, an experimental animal model was developed using administration of carbon tetrachloride (CCl4). Methods: The present study evaluates the hepatoprotective activity of Merremia tridentate plant extract against CCl4 induced liver damage in Wistar albino rats. CCl4 caused a rise in liver and serum AST, ALT, ALP, ACP and total Bilirubin content. Pretreatment and post treatment of rats with ethanolic and aqueous extracts (50,100 & 200 mg/kg b.wt) significantly reduced these serum parameters and antioxidant enzymes levels compared to CCl4 induced rats. The post-treatment had better activity than the pre-treatment groups. Findings: Preliminary phytochemical analysis showed the presence of tannins, saponins, flavonoid, alkaloid, anthocyanin, quinones, glycosides, cardiac glycosides, terpinoids, phenol, coumarin and reducing sugar which may be responsible for the hepatoprotective activity. The liver stabilization activity is due to the presence of bioactive compounds in the Merremia tridentate extracts. Conclusions: Thus, Merremia tridenta extracts ameliorated the liver damage caused by the CCl4and raised the liver and serum enzymes to normal levels:

Keywords: Liver; Detoxification; Carbon Tetrachloride; Merremia trident; Hepatotoxins

References

Tirkey N, Pilkhwal S, Kuhad A, Chopra K. Hasperidin, a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetrachloride in rat liver and kidney. BMC Pharmacol. 2005;5(2). Available from: https://doi.org/10.1186/1471-2210-5-2
Preethi KC, Kuttan R. Hepato and reno protective action of Calendula officinalis L. Flower extract. Indian J. Exp. Biol. 2009;47(3):163–168. Available from: https://pubmed.ncbi.nlm.nih.gov/19405380/#:~:text=Results%20suggest%20a%20protective%20role,lutein%2C%20zeaxanthin%20and%20lycopene%20predominates.
Murugesan GS, Sathishkumar M, Jayabalan R, Binupriya AR, Swaminathan K, Yun SE. Hepatoprotective and Curative Properties of Kombucha Tea Against Carbon Tetrachloride-Induced Toxicity. Journal of Microbiology and Biotechnology. 2009;19(4):397–402. Available from: https://doi.org/10.4014/jmb.0806.374
Bozkurt Y, Bozkurt M, Turkçu G, Sancaktutar AA, Soylemez H, Penbegul N, et al. Caffeic Acid Phenethyl Ester Protects Kidneys against Acetylsalicylic Acid Toxicity in Rats. Renal Failure. 2012;34(9):1150–1155. Available from: https://doi.org/10.3109/0886022x.2012.717485
Weber LWD, Boll M, Stampfl A. Hepatotoxicity and Mechanism of Action of Haloalkanes: Carbon Tetrachloride as a Toxicological Model. Critical Reviews in Toxicology. 2003;33(2):105–136. Available from: https://doi.org/10.1080/713611034
Uhegbu FO, Elekwa I, Ukoha C. Comparative efficacy of crude aqueous extract of <i> Mangiferea Indica, Carica Papaya</i> and Sulphadoxine Pyrimethamine on mice infested with malaria parasite <i> in vivo</i>. Global Journal of Pure and Applied Sciences. 2005;11(3):399–401. Available from: https://doi.org/10.4314/gjpas.v11i3.16523
Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. J. Phytol. 2011;3(12):10–14. Available from: https://updatepublishing.com/journal/index.php/jp/article/view/2737
Mahmoodzadeh Y, Mazani M, Rezagholizadeh L. Hepatoprotective effect of methanolic Tanacetum parthenium extract on CCl4-induced liver damage in rats. Toxicology Reports. 2017;4:455–462. Available from: https://doi.org/10.1016/j.toxrep.2017.08.003
Khan FS, Akram M, Aslam N, Zaheer J, Mustafa SB, Kausar S. Phytochemical analysis and hepatoprotective effect of polyherbal formulation on CCl4 induced hepatotoxicity in mice. Pak. J. Pharm. Sci. 2018;31(6):2719–2793. Available from: https://pubmed.ncbi.nlm.nih.gov/30587485/
Fridous AJ, Islam SNLM, Faruque ABM. Antimicrobial activity of the leaves of Adhatoda vasica, Calotropis gigantea, Nerium odorum and Ocimum sanctum. Bangladesh. J. Botany. 1990;19:227–229. Available from: https://agris.fao.org/agris-search/search.do?recordID=BD9125020
Soladoye MO, Oyesiku O. TO, Lagos AY., eds. Taxonomy of Nigerian Medicinal Plants” in A Textbook of Medicinal Plants from Nigeria. (Vol. 11, pp. 93-149) Lagos University Press. 2008.
Kalaiselvi P, Uma M, Suresh M, Thulasiraman K, Lakshmidevi E. Chronic toxicity studies of aqueous leaf extract of Indian traditional medicinal plant Ocimum tenuiflorum (Linn.) in rats. European Journal of Experimental Biology. 2013;3(5):240–247.
Pierangeli GV, Windell L. Antimicrobial activity and cytotoxicity of Chromolaena odorata (L. f). King and Robinson and Uncaria perrottetii (A. Rich) Merr. extracts. Journal Medicinal Plants Research. 2009;3(7):511–518. Available from: https://doi.org/10.5897/JMPR.9000160
Al-Shabanah O, Tm EH, Aa AM. Effect of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice. Scientia Pharmaceutica. 2002;70(2):135–145. Available from: https://doi.org/10.3797/scipharm.aut-02-16
Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB. Diagnosis and Monitoring of Hepatic Injury. II. Recommendations for Use of Laboratory Tests in Screening, Diagnosis, and Monitoring. Clinical Chemistry. 2000;46(12):2050–2068. Available from: https://doi.org/10.1093/clinchem/46.12.2050
Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB. Diagnosis and Monitoring of Hepatic Injury. I. Performance Characteristics of Laboratory Tests. Clinical Chemistry. 2000;46(12):2027–2049. Available from: https://doi.org/10.1093/clinchem/46.12.2027
Sherman KE. Alanine aminotransferase in clinical practice. A review. Archives of Internal Medicine. 1991;151(2):260–265. Available from: https://pubmed.ncbi.nlm.nih.gov/1992953/
Boyd JW. The Mechanisms Relating to Increases in Plasma Enzymes and Isoenzymes in Diseases of Animals. Veterinary Clinical Pathology. 1983;12(2):9–24. Available from: https://doi.org/10.1111/j.1939-165x.1983.tb00609.x
Potter JD. Cancer prevention: epidemiology and experiment. Cancer Lett. 1997;114:4615–4621. Available from: https://doi.org/10.1016/s0304-3835(97)04615-6
Rice-Evans C, Packer L. Flavonoids in Health and Disease. (pp. 483-522) New York. CRC Press. 1998.
Thanabhorn SK, Jaijoy K, Thamaree S, Ingkaninan K, Panthong A. Acute and subacute toxicity study of the ethanol extract from Lonicera japonica Thunb. Journal of Ethnopharmacology. 2006;107(3):370–373. Available from: https://doi.org/10.1016/j.jep.2006.03.023
Kalaiselvi P, Uma M, Suresh M, Thulasiraman K, Lakshmidevi E. Chronic toxicity studies of aqueous leaf extract of Indian traditional medicinal plant Ocimum tenuiflorum (Linn.) in rats. European Journal of Experimental Biology. 2013;3(5):240–247.
Martin DW, Mayes PA, Rodwell YM. Harper’s Review of Biochemistry. (p. 61) 1981.
Horton R, Moran LA, Ochs R, Rawn JD, Scrimgeour KG. Principles of Biochemistry (2). Prentice Hall. 1996.

Copyright

© 2022 Manimegalai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)