Identification of DNA elements involved in somaclonal variants of Rauvolfia serpentina (L.) arising from indirect organogenesis as evaluated by ISSR analysis

S  Saravanan; R  Sarvesan  and M S  Vinod

doi:10.17485/ijst/2011/v4i10.19

Article

Identification of DNA elements involved in somaclonal variants of Rauvolfia serpentina (L.) arising from indirect organogenesis as evaluated by ISSR analysis

VIEWS 745
PDF 1436

Abstract
Full-Text HTML
Full-Text PDF
How to Cite

Indian Journal of Science and Technology

DOI: 10.17485/ijst/2011/v4i10.19

Year: 2011, Volume: 4, Issue: 10, Pages: 1241-1245

Original Article

Identification of DNA elements involved in somaclonal variants of Rauvolfia serpentina (L.) arising from indirect organogenesis as evaluated by ISSR analysis

S. Saravanan¹ , R. Sarvesan² and M.S. Vinod^3*

¹ Post-graduate & Research Dept. of Plant biology and Biotechnology, Pachaiyappa’s College, Chennai-600030, India
² Post-graduate & Research Dept. of Plant biology and Biotechnology, Presidency College, Chennai-600005, India
³M.S.Swaminathan Research Foundation, Chennai-600 113, India
[email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Rauvolfia serpentina an endangered medicinal plant was chosen for in vitro propagation using modified MS medium. Genetic fidelity study of the regenerated plants were analysed with 18 ISSR markers. A total of 159 monomorphic bands were obtained; one ISSR marker HB-12 showed a polymorphic band among the callus regenerants. The specific polymorphic fragment was then gel eluted, cloned and sequenced.
Keywords: Rauvolfia serpentina, ISSR, Somaclonal variation, Medicinal plant, India