• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2011, Volume: 4, Issue: 10, Pages: 1241-1245

Original Article

Identification of DNA elements involved in somaclonal variants of Rauvolfia serpentina (L.) arising from indirect organogenesis as evaluated by ISSR analysis


Rauvolfia serpentina an endangered medicinal plant was chosen for in vitro propagation using modified MS medium. Genetic fidelity study of the regenerated plants were analysed with 18 ISSR markers. A total of 159 monomorphic bands were obtained; one ISSR marker HB-12 showed a polymorphic band among the callus regenerants. The specific polymorphic fragment was then gel eluted, cloned and sequenced.
Keywords: Rauvolfia serpentina, ISSR, Somaclonal variation, Medicinal plant, India


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