Indian Journal of Science and Technology
Year: 2011, Volume: 4, Issue: 10, Pages: 1241-1245
S. Saravanan1 , R. Sarvesan2 and M.S. Vinod3*
1 Post-graduate & Research Dept. of Plant biology and Biotechnology, Pachaiyappa’s College, Chennai-600030, India
2 Post-graduate & Research Dept. of Plant biology and Biotechnology, Presidency College, Chennai-600005, India
3 M.S.Swaminathan Research Foundation, Chennai-600 113, India
Rauvolfia serpentina an endangered medicinal plant was chosen for in vitro propagation using modified MS medium. Genetic fidelity study of the regenerated plants were analysed with 18 ISSR markers. A total of 159 monomorphic bands were obtained; one ISSR marker HB-12 showed a polymorphic band among the callus regenerants. The specific polymorphic fragment was then gel eluted, cloned and sequenced.
Keywords: Rauvolfia serpentina, ISSR, Somaclonal variation, Medicinal plant, India
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