Indian Journal of Science and Technology
DOI: 10.17485/ijst/2020/v13i10/149934
Year: 2020, Volume: 13, Issue: 10, Pages: 1200 – 1212
Original Article
Nguyen Duc Huy1,*, Le My Tieu Ngoc1, Nguyen Hoang Loc2, Tran Thuy Lan1, Hoang Tan Quang1, Tran Quoc Dung3, Truong Thi Phuong Lan4, Vu Duc Hoang5 and Nguyen Thi Dong Phuong6
1Institute of Biotechnology, Hue University, Hue, 530000, Vietnam
2Faculty of Biology, University of Science, Hue University, 530000, Vietnam
3Faculty of Biology, University of Education, Hue University, 530000, Vietnam
4Faculty of Basic Science, Hue University of Medicine and Pharmacy, Hue University, Hue, 530000, Vietnam
5Faculty of Biology and Environmental Science, University of Science and Education, The University of Danang, Danang, 550000, Vietnam
6Faculty of Chemical Technology and Environment, University of Technology and Education, The University of Danang, Danang, 550000, Vietnam
*Author for correspondence:
Nguyen Duc Huy
Institute of Biotechnology, Hue University, Hue, 530000, Vietnam
E-mail ID: ndhuy@hueuni.edu.vn
Objective: The present study reported the isolation of lactic acid bacteria from the shrimp gastrointestinal tract and evaluation of its antagonistic activity against pathogenic bacteria.
Methods/ statistical analysis: Lactic bacteria from the gastrointestinal tract of alive healthy shrimp were screened on sterilized MRS medium. Vibrio sp. inhibition by isolates was conducted by the agar-well diffusion assay. The isolates were identified by 16S rRNA sequencing. Antibacterial activity was investigated against 25 Vibrio sp. isolates, Staphylococcus aureus ATCC 25023, and Escherichia coli ATCC 85922. Hemolytic activity, salt, and antibiotic resistance were also evaluated.
Findings: Total of 22 isolates exhibited Vibrio parahaemolyticus inhibition with antagonistic activities ranged from 220 AU/ml to 460 AU/ml. Sequence analysis of 16S rRNA indicated the 5 isolates belong to Weissella cibaria species, namely HN02, HN03, HN04, and HN06 AND HN07. Among these, W. cibaria HN05 had highest antagonistic activity and exhibited the broad inhibition spectrum against Vibrio sp. as well as E. coli ATCC 85922 but not S. aureus ATCC 25023. β-hemolytic and salt resistance assays showed that W. cibaria HN05 had no β-hemolytic activity and grew well in medium containing 5% of salt. Antibiotic resistance analysis indicated W. cibaria HN05 was suppressed by ampicillin, chloramphenicol, oxytetracycline hydrochloride, and chlortetracycline hydrochloride.
Application/improvements: This is the first study on isolation and evaluation pathogenic bacteria including Vibrio sp. inhibition by W. cibaria, which is isolated from shrimp gastrointestinal tract.
Keywords: Antagonistic Activity, Gastrointestinal Tract, Shirmp, Weissella cibaria, Vibrio sp.
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