Mean Expression Value Normalized and Absolute Quantified miR-21 Found to be a Potential Diagnostic and Prognostic Marker for IgA Nephropathy

Santosh Kumar; C  Priscilla; Sreejith Parameswaran; Deepak Gopal Shewade; A  Charan Goud; Rajesh Nachiappa Ganesh

doi:10.17485/ijst/2020/v013i09/149977

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Mean Expression Value Normalized and Absolute Quantified miR-21 Found to be a Potential Diagnostic and Prognostic Marker for IgA Nephropathy

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2020/v013i09/149977

Year: 2020, Volume: 13, Issue: 9, Pages: 1078-1088

Original Article

Mean Expression Value Normalized and Absolute Quantified miR-21 Found to be a Potential Diagnostic and Prognostic Marker for IgA Nephropathy

Santosh Kumar¹, C. Priscilla¹, Sreejith Parameswaran², Deepak Gopal Shewade³, A. Charan Goud³, Rajesh Nachiappa Ganesh^1,*

¹Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605006, India
²Department of Nephrology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605006, India
³Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605006, India

^*Author for correspondence:
Rajesh Nachiappa Ganesh
Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605006, India
E-mail ID: [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background/objectives: miR-21 has been linked to renal fibrosis and miR-155 too has shown different expression in IgAN whose pathogenesis is partially understood. In this study, we explored the utility of miR-21 and miR-155 in IgA nephropathy.

Methods: We recruited 40 IgA nephropathy (IgAN) patients and 15 healthy controls (HC). 2 ml blood sample was collected from each study participant. Mean expressed value normalization and absolute and relative quantification methods were used for quantitative polymerase chain reaction (PCR) experiment for miRNA expression. Logistic regression analysis was performed for diagnostic and prognostic value of miR-21.

Findings: Mean expressed value normalization reduced the variation in groups and between groups without changing the mean value. miR-155 showed similar expression in IgAN and healthy controls. There was three-fold increases in miR-21 in IgAN than the HC. miR-21 was able to differentiate the mesangial hypercellularity and tubular atrophy/interstitial fibrosis stages with sensitivity 84 and 70 percent, respectively.

Applications: miR-155 can be used as a reference control gene for IgAN on quantitative PCR-based experiments. miR-21 can be used as a diagnostic and prognostic marker for IgAN.

Keywords: miR-21, miR-155, IgAN, Tubular Atrophy, Interstitial Fibrosis.