Indian Journal of Science and Technology

Abstract

Peptide obtained from skin of Rana tigerina is a small, cationic peptide with antimicrobial activity and recently also reported for their anti cancerous and immunomodulatory properties. Here, we present the immunomodulatory effect of skin peptide from R. tigerina on the antigen presentation activity of macrophages and its correlations in induction of strong DTH response. 2, 4-dinitro-1-flurobenzene (DNFB) sensitized tumour bearing and normal mice were injected with skin peptide of R. tigerina to examine appearance of DTH response. Findings of present study showed that the skin peptides from R. tigerina augment antigen presenting activity of macrophages and maximum DTH response occurred at 48th hrs after antigenic challenge. This study also suggest that the PKC plays major role behind the augmentation of macrophage activity and in turn DTH reaction, as observed by enhanced PKC translocation from cytosol towards membrane. Present endeavor suggests that this peptide can potentially enhance cell mediated immune response. These findings can be used to construct synthetic peptides with desirable modifications to fight cancer and other therapeutic conditions.
Keywords: Antigen Presenting Cell, DNFB, DTH, Frog Skin peptide, T-cells, Rana tigerina.