Indian Journal of Science and Technology
Year: 2023, Volume: 16, Issue: Special Issue 1, Pages: 30-38
C Zothansanga1, Khawlhring Lalchhandama1,2, Lalramliana1*, Malsawmdawngzuali Tara1, Betsy Zodinpuii2, John Zothanzama3
1Department of Life Sciences, Pachhunga University College, Aizawl, Mizoram, India
2Department of Zoology, Pachhunga University College, Aizawl, Mizoram, India
3Department of Biotechnology, Mizoram University, Aizawl, Mizoram, India
Email: [email protected]
Received Date:23 January 2023, Accepted Date:09 June 2023, Published Date:30 July 2023
Objectives: The present study aims to validate the presence of Chalcosoma atlas in Mizoram, India, and to provide morphological and molecular descriptions to reveal sexual dimorphism for future reference. Methods: Chalcosoma atlas was sampled during monsoon season in 2021 near Palak lake, Mizoram. Specimens were collected using light traps at night and were further preserved in 70% alcohol. Species identification and morphological measurements were done along with the extraction of male and female genitalia. Statistical analysis was performed using SPSS version 28.0. Student’s T-test and Pearson’s correlation were performed to determine significant differences between various morphological characters. The molecular data for the species was generated by performing maximum likelihood phylogenetic analysis using a partial mitochondrial 16S rRNA gene. Findings: Four male and two female Chalcosoma atlas were collected. The collected samples were measured using a slide calliper and their morphological characters along with the differences in male and female were recorded. The molecular analysis using the 16S rRNA gene shows that all the collected samples show similarity (99%) with the database sequence of C. atlas. Analysis using Student’s T-test shows that some characters of male and female have a significant relationship. Pearson’s correlation shows that there is a significant positive relationship between different characters in both males and females while a significant negative correlation was observed only between some female characters. Male and female genitalia were taken out and photographed as they also serve as an important differentiating character. Novelty: Chalcosoma atlas was previously reported from Mizoram, India. However, this study validates the occurrence of C. atlas in Mizoram, India with detailed morphological and molecular descriptions required for the identification of the species and diagnosis of sexual dimorphism between male and female C. atlas.
Keywords: Correlation; Genitalia; Occurrence; Sexual dimorphism; Bio diversity hotspot
© 2023 Zothansanga et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)
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