Indian Journal of Science and Technology
Year: 2021, Volume: 14, Issue: 38, Pages: 2888-2898
S Chaitanya Kumari1, V Selvakumar2, P Naga Padma1, K Anuradha1*
1Department of Microbiology, Bhavan’s Vivekananda College of Science, Humanities & Commerce, Sainikpuri, Sec-bad, 500094, Telangana, India
2Department of Maths and Statistics, Bhavan’s Vivekananda College of Science, Humanities & Commerce, Sainikpuri, Sec-bad, 500094, Telangana, India
Email: [email protected]
Received Date:11 June 2021, Accepted Date:16 September 2021, Published Date:15 November 2021
Background/Objectives: The main objective of the present study is to screen an efficient plant extract sources for the green synthesis of silver nanoparticles (AgNPs) and optimization of various conditions for the synthesis using a statistical methodology like Taguchi design. The antimicrobial activity of the synthesized AgNPs against common wound causing dermal pathogens was also checked. Methods: The conditions for green synthesis, such as the concentration of silver nitrate, amount of plant extract, synthesis reaction time, temperature, and pH of the plant extract, were optimized by using Taguchi design (L27type). Ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), and Transmission electron microscopy (TEM) were used for the characterization of produced green AgNPs. The antibacterial activity of the obtained green AgNPs was tested against most common wound forming dermal pathogens like S1(Staphylococcus aureus-Multi drug-resistant, hospital strain), S2 (Staphylococcus aureus-pimple source), S3 (Staphylococcus aureuswound sample), S4 (Pseudomonas sp.-hospital sample), and S5 (Pseudomonas sp.-wound sample) using Agar well diffusion method. Findings: Thirty different aqueous leaf extracts were screened for AgNPs using a flask level unidimensional study. Among all, the aqueous leaf extract of Aloe vera was found to be the most suitable for the green synthesis of AgNPs. The optimum conditions for the green synthesis of AgNPs were observed as pH- 7.0, temperature 35◦C, 10mM silver nitrate (AgNO3), 50% (v/v) plant extract for a time period of 30 min. Novelty/Applications: The Taguchi statistical method was used for the optimization of the green synthesis of AgNPs with suitable experiential tests. Finally, this rapid and easy process of green synthesis of AgNPs using aqueous leaf extract of Aloe vera demonstrated broad spectrum activity as an antibacterial agent against the wound-causing dermal pathogens in the medical field by exhibiting the promising minimum inhibitory concentration (MIC) of AgNPs as 0.0535mg/L.
Keywords: Green synthesis; silver nanoparticles; Statistical optimization; Taguchi method Antibacterial activity
© 2021 Kumari et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)
Subscribe now for latest articles and news.