• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2021, Volume: 14, Issue: 25, Pages: 2111-2118

Original Article

Physicochemical properties of Honey from Traditional Beekeepers of Sohra

Received Date:09 June 2021, Accepted Date:02 July 2021, Published Date:18 July 2021


Objective: To determine the physicochemical properties of honey to help ascertain its quality. The properties investigated in the present work are specific gravity, refractive index, moisture content, total solids, pH, free acidity, electrical conductivity, and hydroxymethylfurfural (HMF). Methods/Statistical analysis: To determine properties of honey such as refractive index, moisture content, pH, free acidity, electrical conductivity, and hydroxymethylfurfural, the procedures prescribed by the International Honey Commission were followed. Specific gravity was determined using the method described by Rebiai et al. Total solids was calculated by the formula given by Amin et al. Statistical analyses were carried out in Microsoft Excel 2016, and all readings were presented as mean±standard deviation. Correlation studies were also used wherever applicable. Findings: The moisture content lies in the range (16.6±0.4) g/100g to (19.4±0.3) g/100g. The total solids of all honey samples were above 80%. The pH varies from (3.59±0.02) to (3.73±0.01). The free acidity recorded a minimum of (32.3±1.5) meq/kg and a maximum of (42.0±2.0) meq/kg. The honey samples recorded electrical conductivities between (0.51±0.15) mS/cm and (0.61±0.25) mS/cm. The HMF varies from (8.9±0.2)mg/kg to (34.2±0.2) mg/kg. All the properties that were measured were well within the range prescribed by international agencies responsible for the quality control of Honey. Novelty: Adulteration of honey is a major concern as it affects the health and well being of a person. The recent study by the Centre for Science and Environment, New Delhi which reported adulteration of honey by eight household brands in the Indian market is a cause of alarm. A noteworthy feature of the current study was that it highlighted the superior quality of honey from traditional bee-keepers of Sohra, which even have the potential for export. No adulteration was detected relative to the parameters being investigated.


Sohra, Bee, Honey, Physicochemical properties, Quality, Adulteration


  1. Alvarez-Suarez JM, Tulipani S, Díaz D, Estevez Y, Romandini S, Giampieri F, et al. Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compoundsFood and Chemical Toxicology2010;48(8-9):24902499. Available from: https://dx.doi.org/10.1016/j.fct.2010.06.021
  2. Fakhlaei R, Selamat J, Khatib A, Razis AFA, Sukor R, Ahmad S, et al. The Toxic Impact of Honey Adulteration: A ReviewFoods2020;9(11). Available from: https://dx.doi.org/10.3390/foods9111538
  3. Barrientos AH, Morales AEV, Callejas LPA, Cabrera MAS, López EG, Ayala EG. Sustainable Beekeeping Cooperative Societies: The Case of Mexico CityInternational Journal of Food Science and Agriculture2021;5(1):7684. Available from: https://dx.doi.org/10.26855/ijfsa.2021.03.011
  4. Mahnot NK, Saikia S, Mahanta CL. Quality characterization and effect of sonication time on bioactive properties of honey from North East IndiaJournal of Food Science and Technology2019;56(2):724736. Available from: https://dx.doi.org/10.1007/s13197-018-3531-1
  5. Rebiai A, Lanez T. Comparative Study of Honey Collected from Different Flora of AlgeriaJournal of Fundamental and Applied Sciences2015;6(1):4855. Available from: https://dx.doi.org/10.4314/jfas.v6i1.4
  6. Chataway HD. The Determination of Moisture in HoneyCanadian Journal of Research1932;6(5):532547. Available from: https://dx.doi.org/10.1139/cjr32-041
  7. Amin WA, Safwat M, El-Iraki SM. Quality criteria of treacle (black honey) Food Chemistry1999;67:1720. Available from: https://dx.doi.org/10.1016/s0308-8146(99)00086-2
  8. White J. Spectrophotometric method for hydroxymethylfurfural in honeyJournal of the Association of Official Analytical Chemists1979;62:509514. Available from: 10.1093/jaoac/62.3.509
  9. Sunkesula MSB, Reddy MS. Physico-biochemical and Bioactive properties of honey samples from Southern IndiaJournal of Advanced Scientific Research2021;12(1):8387.
  10. Krishnasree V, Ukkuru PM. Quality Analysis of Bee HoneysInternational Journal of Current Microbiology and Applied Sciences2017;6(2):626636. Available from: https://dx.doi.org/10.20546/ijcmas.2017.602.071
  11. Albu A, Radu-Rusu CG, Pop IM, Frunza G, Nacu G. Quality Assessment of Raw Honey Issued from Eastern RomaniaAgriculture2021;11(3). Available from: https://dx.doi.org/10.3390/agriculture11030247
  12. Bouhlali EdT, Bammou M, Sellam K, Midaoui AE, Bourkhis B, Ennassir J, et al. Physicochemical properties of eleven monofloral honey samples produced in MoroccoArab Journal of Basic and Applied Sciences2019;26(1):476487. Available from: https://dx.doi.org/10.1080/25765299.2019.1687119
  13. Khan KA, Al-Ghamdi AA, Ansari MJ. The characterization of blossom honeys from two provinces of PakistanItalian Journal of Food Science2016;28(4):625638.
  14. Nedji N, Loucif-Ayad W. Antimicrobial activity of honey samples from different sites in Northeast AlgeriaLife Science Journal2021;18(1):7379. Available from: 10.7537/marslsj180121.10
  15. Sohaimy SAE, Masry SHD, Shehata MG. Physicochemical characteristics of honey from different originsAnnals of Agricultural Sciences2015;60(2):279287. Available from: https://dx.doi.org/10.1016/j.aoas.2015.10.015
  16. Moniruzzaman M, Sulaiman SA, Khalil MI, Gan SH. Evaluation of physicochemical and antioxidant properties of sourwood and other Malaysian honeys: a comparison with manuka honeyChemistry Central Journal2013;7(1):112. Available from: https://dx.doi.org/10.1186/1752-153x-7-138
  17. MS, Kumar A. Physiochemical and mineral analysis of Honeysamples from varanasi districtInternational Journal of Pharma and Bio Sciences2017;8(2):160166. Available from: https://dx.doi.org/10.22376/ijpbs.2017.8.2.b160-166
  18. Bogdanov S, Lüllmann C, Martin P, Ohe Wvd, Russmann H, Vorwohl G, et al. Honey quality and international regulatory standards: review by the International Honey CommissionBee World1999;80(2):6169. Available from: https://dx.doi.org/10.1080/0005772x.1999.11099428
  19. Standard for Honey, CXS 12-1981 1, Adopted in 1981Codex Alimentarius Commission2001.
  20. Abdulkhaliq A, Swaileh KM. Physico-chemical properties of multi-floral honey from the West Bank, PalestineInternational Journal of Food Properties2017;20(2):447454. Available from: https://dx.doi.org/10.1080/10942912.2016.1166128
  21. Kumar A, Gill JPS, Bedi JS, Manav M, Ansari MJ, Walia GS. Sensorial and physicochemical analysis of Indian honeys for assessment of quality and floral originsFood Research International2018;108:571583. Available from: https://dx.doi.org/10.1016/j.foodres.2018.04.005
  22. Israili ZH. Antimicrobial Properties of HoneyAmerican Journal of Therapeutics2014;21(4):304323. Available from: https://dx.doi.org/10.1097/mjt.0b013e318293b09b
  23. Islam A, Khalil I, Islam N, Moniruzzaman M, Mottalib A, Sulaiman SA. Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one yearBMC Complementary and Alternative Medicine2012;12(1). Available from: https://dx.doi.org/10.1186/1472-6882-12-177
  24. Rane MD, Doke K. Physicochemical Properties, Antioxidant Features of Honey Samples from Ecological Niches of Western MaharashtraInternational Journal of Science and Research2014;3(8):17231727.
  25. Almasi R, Sekarappa B. Analysis of unifloral and multifloral honey for physicochemical properties in Southern KarnatakaIndia. International Journal of Recent Scientific Research2019;10(5):3246932473. Available from: http://dx.doi.org/10.24327/ijrsr.2019.1005.3477
  26. Piazza MG, Accorti M, Oddol P. Electrical conductivity, ash, colour and specific rotatory power in Italian unifloral honeysApicoltura1991;7:5163.
  27. Stefan B. The Book of HoneyChapter 5, Honey Composition.2016. Bee Product Science. Available from: https://www.researchgate.net/publication/304011775_Honey_Composition


© 2021 Kharkamni. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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