Profiling of Metabolites from Human Intervertebral Disc through Gas Chromatography - Mass Spectrometry

R  Veera Ranjani; N  Senthil; M  Raveendran; S  Vellaikumar; R  Gnanam; M  K  Rishi  and S  Rajasekaran

doi:10.17485/ijst/2014/v7i8.24

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Profiling of Metabolites from Human Intervertebral Disc through Gas Chromatography - Mass Spectrometry

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2014/v7i8.24

Year: 2014, Volume: 7, Issue: 8, Pages: 1228–1235

Original Article

Profiling of Metabolites from Human Intervertebral Disc through Gas Chromatography - Mass Spectrometry

R. Veera Ranjani¹ , N. Senthil^1*, M. Raveendran¹ , S. Vellaikumar¹ , R. Gnanam¹ , M. K. Rishi² and S. Rajasekaran²

1 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultutal University, Coimbatore - 641003, Tamil Nadu, India; rajaranji@gmail.com, senthil_natesan@tnau.ac.in, raveendrantnau@gmail.com, vellai1973@gmail.com, rgnanam2000@yahoo.com
2 Department of Orthopaedics and Spine Surgery, Ganga Hospital, Coimbatore, Tamil Nadu, India; rishiortho@gmail.com, rajasekaran.orth@gmail.com

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

This work aims to identify the metabolites present in the human Intervertebral Disc (IVD). Metabolomic analysis of human IVD tissue has not been extensively done to date. Knowledge on the metabolites present in the IVD tissue in humans is very limited and many compounds are yet to be identified. In this study, we have carried out the metabolic profiling for human IVD through Gas Chromatography/Mass Spectrometry (GC/MS). This is the first initial study that has compared the metabolites of control and diseased IVD. We have identified 75 different chemical compounds in IVD, and also metabolites that are unique to the diseased IVD, suggesting that some of these metabolites might play a role in disc degenerative disease.

Keywords: DDD, Human, Intervertebral Disc, Metabolite Profiling