• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2008, Volume: 1, Issue: 7, Pages: 1-6

Original Article

Purification, characterization of alkaline protease enzyme from native isolate Aspergillus niger and its compatibility with commercial detergents


An alkaline protease producing strain Aspergillus niger was isolated from local soil samples and enzyme production was optimized under submerged conditions. Maximum enzyme production of the culture occurred in mesophilic temperature 45°C and pH 8.5. Glucose and ammonium sulfate proved to be the best carbon and nitrogen sources respectively. The molecular weight of the enzyme determined by SDS-PAGE was found to be 38 kDa. The enzyme acted optimally at pH 10 and 50°C. It was thermo stable and retained full activity even at the end of 1 hour of incubation at 40°C. It was inhibited by Cu++, Hg++, Zn++, EDTA and sodium azide. The enzyme retained more than 50% activity after 60 min incubation at 40°C in the presence of detergents such as Tide, Surf, Wheel and Henko indicating its suitability for application in detergent industry.


Keywords: Alkaline protease, Aspergillus niger, purification, characterization, detergent.


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