Indian Journal of Science and Technology
DOI: 10.17485/ijst/2008/v1i7.8
Year: 2008, Volume: 1, Issue: 7, Pages: 1-6
Original Article
M. Kalpana Devi, A. Rasheedha Banu, G.R. Gnanaprabhal, B.V. Pradeep and M. Palaniswamy
Department of Microbiology, Karpagam University, Coimbatore 641 021, Tamil Nadu, India. [email protected]
*Author for the correspondence:
M. Palaniswamy
Department of Microbiology, Karpagam University
Coimbatore 641 021, Tamil Nadu, India.
E-mail: [email protected]
An alkaline protease producing strain Aspergillus niger was isolated from local soil samples and enzyme production was optimized under submerged conditions. Maximum enzyme production of the culture occurred in mesophilic temperature 45°C and pH 8.5. Glucose and ammonium sulfate proved to be the best carbon and nitrogen sources respectively. The molecular weight of the enzyme determined by SDS-PAGE was found to be 38 kDa. The enzyme acted optimally at pH 10 and 50°C. It was thermo stable and retained full activity even at the end of 1 hour of incubation at 40°C. It was inhibited by Cu++, Hg++, Zn++, EDTA and sodium azide. The enzyme retained more than 50% activity after 60 min incubation at 40°C in the presence of detergents such as Tide, Surf, Wheel and Henko indicating its suitability for application in detergent industry.
Keywords: Alkaline protease, Aspergillus niger, purification, characterization, detergent.
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