Indian Journal of Science and Technology
DOI: 10.17485/ijst/2017/v10i24/113409
Year: 2017, Volume: 10, Issue: 24, Pages: 1-7
Original Article
P. Gayatri1 *, M. Ajitha2 , Y. Madhusudan Rao1 and P. Pavan Kumar3
1Department of Pharmaceutical Sciences, Vaagdevi Institute of Pharmaceutical Sciences, Bollikunta – 506005, Warangal, Telangana, India; [email protected] 2Centre for Pharmaceutical Sciences, IST, JNTUH, Hyderabad – 500072, Telangana, India 3Dr. Reddy’s Laboratories Limited, Bachupally, Hyderabad – 500090, Telangana, India
*Author for the correspondence:
P. Gayatri
Department of Pharmaceutical Sciences, Vaagdevi Institute of Pharmaceutical Sciences, Bollikunta – 506005, Warangal, Telangana, India; [email protected]
Objective: A sensitive and précised bioanalytical method for quantification of Thiocolchicoside (THC) in rabbit serum was developed using high performance liquid chromatographic technique. Aceclofenac (ACE) was used as Internal Standard (IS). Methods: Phenomenix Luna® C18 column (150 x 4.6mm.i.d.5 µ) was used for method development of THC.Ammonium di hydrogen phosphate buffer: Acetonitrile (60:40) was used as mobile phase where in pH was adjusted to 4. 5 with glacial acetic acid. Findings: Flow rate was set to 1.0 mL/min and elements were monitored at 264 nm by UV detector. The retention times detected were 3.16 and 4.25 minutes for THC & ACE respectively. Linearity of the calibration curve was in the range of 10- 4000 ng mL-1. Inter day, intra-day coefficient of variation and percent error values of the developed assay method was with the acceptable limits of less than 15 %. The mean recovery was recorded to be more than 98.6 and 99.5 % for THC and ACE respectively. Application: Validated bio-analytical method was successfully applied for estimation of THC in Rabbit serum after application of Transdermal films to the rabbit skin.
Keywords: Pharmacokinetics, Rabbit Serum, Thiocolchicoside, Transdermal films
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