Indian Journal of Science and Technology
Year: 2015, Volume: 8, Issue: 14, Pages: 1-7
Trupti K. Ashar1*, V. W. Rajasekar2 and Anupama Datla2
1 Department of Management, AMET University, Chennai, India; [email protected]
2 R&D Biotech Division, Fermenta Biotech Ltd, DIL Complex, Majiwada, Thane, 400610, India; [email protected]
Beta-lactam acylase enzyme producers were isolated from soil samples. Soil samples were induced with phenyl amino acid derivatives for selelctive isolation. Out of the two potential isolates obtained, one of the isolates was studied for presence of beta-lactam acylase enzyme with cephalexin as model substrate. Enzyme activity of isolate grown in media at varying pH showed it to be tolerant to alkaline pH 8.5. Also the substrate specificity for various cephalosporins showed it most selective to cephalexin.
Keywords: Betalactam Acylase Enzyme, Cephalexin, Soil Isolate
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