• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2015, Volume: 8, Issue: 14, Pages: 1-7

Original Article

Screening of Beta-lactam Acylase Producers from Soil and Characterization of Isolates for Substrate Specificity for Cephalosporins


Beta-lactam acylase enzyme producers were isolated from soil samples. Soil samples were induced with phenyl amino acid derivatives for selelctive isolation. Out of the two potential isolates obtained, one of the isolates was studied for presence of beta-lactam acylase enzyme with cephalexin as model substrate. Enzyme activity of isolate grown in media at varying pH showed it to be tolerant to alkaline pH 8.5. Also the substrate specificity for various cephalosporins showed it most selective to cephalexin.

Keywords: Betalactam Acylase Enzyme, Cephalexin, Soil Isolate


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