• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2023, Volume: 16, Issue: Special Issue 1, Pages: 10-18

Original Article

Study of the Mizo Medicinal Plant, Helicia excelsa on its Phytochemical Components, Antioxidant Property and Antibacterial Activity

Received Date:23 January 2023, Accepted Date:09 June 2023, Published Date:30 July 2023


Objectives: Helicia excelsa (Roxb.) Blume (family Proteaceae) is an evergreen tree of ethno-medicinal importance among the Mizo people of India. The study was planned to unveil the chemical and antibacterial properties. Methods: A petroleum ether extract of H. excelsa leaf, prepared by Soxhlet extraction, was used to investigate quantitative phytochemical properties using nine different tests. Qualitative antioxidant tests such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging, total antioxidant, total flavonoid, total phenolic content and reducing power assays were performed using spectrophotometric analysis. Agar well diffusion method was used to study the antibacterial activity against Gram-positive and Gram-negative species. Findings: Phytochemical detection tests showed that the plant extract contained alkaloids, amino acids, carbohydrates, flavonoids, glycosides, phenols, phytosterols, proteins, and tannins, while saponin was absent. DPPH scavenging assay indicated that the plant extract had an IC50 value of 34.122.51. Reducing power assay indicated a concentration-dependent activity on the substrate, potassium ferricyanide. Total antioxidant content showed 9.090.321 mg/g ascorbic acid equivalent (AAE), while total phenolic and total flavonoid content have 2.430.039 mg/g quercetin equivalent (QE) and 47.62.757 mg/g gallic acid equivalent (GAE), respectively. Positive antibacterial activity was seen against Gram-positive Staphylococcus aureus and Gram-negative Salmonella typhimurium. Novelty: The study showed promising results for H. excelsa as a valuable medicinal plant that contains bioactive compounds and exhibits antibacterial activity.

Keywords: Helicia excelsa; antibacterial activity; antioxidant activity; bioactive compound; medicinal plant


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© 2023 Tlau et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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