Indian Journal of Science and Technology

Abstract

Quantitative characteristics of interphase distribution of aromatic amino acids were obtained under identical conditions; a general framework for the analysis was developed including preliminary extraction of analytes from aqueous salt solutions using an extractant based on Poly-N-Vinyl Pyrrolidone. Application of ammonium sulfate as a salting-out agent proved to be feasible. Spectral analysis methods allowed establishing the structure of complexes formed during amino acids extraction. The structure of distributed complexes was determined and the mechanisms of the interaction of amino acids with the polymer were proposed based on the data concerning characteristic absorption band displacement in the IR spectra of the complexes. Conditions for almost complete extraction of phenylalanine, tyrosine and tryptophan from aqueous salt solutions were optimized. The method for extraction-spectrophotometric determination of aromatic amino acids in aqueous solution was developed. The studied extraction systems are applicable for almost complete extraction of phenylalanine, tyrosine and tryptophan from aqueous solutions during the analysis of various genesis media, including food products and pharmaceuticals. 
Keywords: Absorption Spectra, Aromatic Amino Acids, Extraction, Poly-N-Vinyl Pyrrolidone, UV and IR Spectroscopy