A method for small scale genomic DNA isolation from chickpea (Cicer arietinum L.) suitable for molecular marker analysis

Neha Joshi; Anamika Rawat; R  B  Subramanian and K  S  Rao

doi:10.17485/ijst/2010/v3i12.6

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A method for small scale genomic DNA isolation from chickpea (Cicer arietinum L.) suitable for molecular marker analysis

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2010/v3i12.6

Year: 2010, Volume: 3, Issue: 12, Pages: 1214-1218

Original Article

A method for small scale genomic DNA isolation from chickpea (Cicer arietinum L.) suitable for molecular marker analysis

Neha Joshi, Anamika Rawat, R. B. Subramanian and K. S. Rao ^*

B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, India
[email protected] , [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

We describe a DNA isolation procedure for chickpea (Cicer arietinum L.) which is rapid and less expensive without involving ultra centrifugation or column purification steps. DNA preparation obtained from the present study was essentially suitable for PCR analysis which is one of the key steps in crop improvement programme through marker development and genetic engineering techniques. The yield of DNA ranged from 0.595- 5.550 μg/ml and the purity ratio was between 1.025- 2.010 indicating minimum levels of contaminating metabolites. The present protocol offers as a reliable, and consistent DNA isolation method for chickpea that yields large amount of pure & intact DNA.
Keywords: DNA isolation, Cicer arietinum, PCR analysis.