Clinical Evaluation of Automated BACTEC MGIT 960 System for Identification, Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis Clinical Isolates

Thirumurugan Ravibalan; Antony V  Samrot; Kathirvel Maruthai; Vallayyachari Kommoju; Surendar Kesavan; Muthuraj Muthaiah

doi:10.17485/ijst/2016/v9i8/76444

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Clinical Evaluation of Automated BACTEC MGIT 960 System for Identification, Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis Clinical Isolates

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2016/v9i8/76444

Year: 2016, Volume: 9, Issue: 8, Pages: 1-5

Original Article

Clinical Evaluation of Automated BACTEC MGIT 960 System for Identification, Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis Clinical Isolates

Thirumurugan Ravibalan^1,2, Antony V. Samrot², Kathirvel Maruthai¹ , Vallayyachari Kommoju¹ , Surendar Kesavan¹ , Muthuraj Muthaiah^1*

¹Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry - 605006, India. ²Department of Biotechnology, Sathyabama University, Rajiv Gandhi Road, Jeppiaar Nagar, Chennai - 600119, Tamilnadu, India; [email protected]

*Author for Correspondence
Muthuraj Muthaiah Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry - 605006, India.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background and Objectives: The objective of this study was to evaluate the clinical applicability of BACTEC Mycobacterial Growth Indicator Tube 960TM system with Lowenstein-Jensen (LJ) culture for routine recovery and drug susceptibility test analysis of mycobacteria from clinical specimens. Method and Statistical Analysis: One hundred and seven clinical specimens were processed by routine laboratory procedures like p-nitro benzoic acid test and PCR amplification of IS6110 and inoculated in LJ medium and MGIT for mycobacterial growth recovery and DST procedures. Findings: Ninety four (87.8%) samples were demonstrated reproducible result by MGIT, in which 89 (83.1%) were smear positive and all the specimens were confirmed as M. tuberculosis complex by MGIT PNB and IS6110 PCR procedures. The observed mean time for mycobacterial detection was 9 days for primary culture and 11 days for DST in MGIT system. Applications/ Improvements: The use of BACTEC MGIT 960 system was found to be a sensitive, rapid mycobacterial recovery and culture system and offers precise identification and detection of drug resistance from clinical isolates at earliest.