• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2008, Volume: 1, Issue: 3, Pages: 1-13

Original Article

Differential expression of PML in 60Co γ-ray and γ-IFN- induced apoptosis in B-lymphocytes.


The function of PML in apoptosis was elucidated in human B lymphocyte- derived cell line. For this purpose, the number of the PML focus and the percentage of apoptotic cells were determined periodically with immunofluorescent staining after 60Coγ-ray or γ-interferons (γ-IFN) treatment. On irradiation with 60Coγ-ray or treatment with γ-IFN, PML protein was over-expressed. This was measured from PML foci, which peaked at 72 hr and 24 hr, respectively. The B-cell line also contained a greater proportion of apoptotic cells after the treatments. The strongest induction of apoptosis both by 60Co-γ ray irradiation and by γ-IFN treatment was observed 24 hr later than the induction of PML expression. In addition, γ- rays-induced apoptosis and PML expression were mediated through caspase-8 but not through caspase-3. However, caspase-8 was involved in γ-IFN-induced PML expression and apoptosis. While caspase-3 is involved solely in PML expression, and partially in apoptosis. These results suggest that 60Co-γ ray irradiation or γ-IFN treatment can induce PML protein expression and apoptosis in the B-cell line as caspase-3 dependently or independently.

Key words: PML Protein, Apoptosis, 60Co-γ ray, γ-IFN, Caspase activation. 


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