Indian Journal of Science and Technology
Year: 2022, Volume: 15, Issue: 7, Pages: 309-317
V Ramamurthy1*, T A K Mumtaz Begum2
1Head, PG & Research Department of Biochemistry, Marudupandiyar College, (Affiliated to Bharathidasan University), Thanjavur, 613403, Tamil Nadu, India
2Research scholar, Department Of Biochemistry, Marudupandiyar College, (Affiliated to Bharathidasan University), Thanjavur, 613403, Tamil Nadu, India
Email: [email protected]
Received Date:07 December 2021, Accepted Date:28 January 2022, Published Date:25 February 2022
Objectives: To determine the mode of hypolipidemic action of Hulled Barley Grains Powder (HBGP) on the gene level expression of PPAR-g and SIRT1 in High-Fat Diet (HFD) induced hyperlipidemic rats. Methods: The HBGP was subjected to High-Performance Liquid Chromatography (HPLC) to quantify the major phytochemicals. Male Sprague Dawley rats were fed an HFD for 14 weeks to induce hyperlipidemia. The rats in groups 1 and 2 were fed a standard diet. Group 3 and 4 rats were fed HFD for 14 weeks. From the third week onwards, Group 2 and 4 rats received 50% of HBGP mixed in the feed, in addition to 50% of the normal and HFD diets. The rats were euthanized after 14 weeks, and plasma lipids, serum levels of adiponectin and leptin were measured. The mRNA expression of PPAR-g and SIRT1were also measured. Findings: HBGP was found to contain phenolics: Ferulic acid-9.11, benzoic acid-3.58, cinnamic acid-1.191, vanillic acid-2.43, flavonoids: quercetin- 6.53, kaempferol-1.04, and myricetin-1.443 (mg/g). HFD+HBGP fed rats showed a significant decrease in blood lipids and leptin levels. Furthermore, serum adiponectin levels and mRNA expression of PPAR-g and SIRT1 were found to be quantitatively increased in HBGP co-administered rats when compared to HFD fed rats. Novelty: HBGP exhibits hypolipidemic activity by elevating the mRNA expression of PPAR-g and SIRT1. The presence of various phytochemicals such as ferulic acid, quercetin, benzoic acid, vanillic acid, myricetin, cinnamic acid, and kaempferol might be accounted for the hypolipidemic effect of HBGP.
Keywords: Hyperlipidemia; HFD; HBGP; HPLC; Adiponectin; Leptin; PPAR-g ; SIRT1
© 2022 Ramamurthy & Begum.
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