Genetic diversity and toxic genes analysis of vibrio spp. isolated from white leg shrimp and marine fishes cultured in Tam G iang lagoon in Thua Thien Hue province, Vietnam

Hoang Tan Quang; Tran Thuy Lan; Truong Thi Hong Hai; Pham Thi Hai Yen; Tran Quang Khanh Van; Ho Thi Tung; Mac Nhu Binh; Nguyen Khoa Huy Son; Nguyen Quang Linh; Nguyen Duy Quynh Tram

doi:10.17485/IJST/v13i13.161

Article

Genetic diversity and toxic genes analysis of vibrio spp. isolated from white leg shrimp and marine fishes cultured in Tam G iang lagoon in Thua Thien Hue province, Vietnam

VIEWS 1914
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Indian Journal of Science and Technology

DOI: 10.17485/IJST/v13i13.161

Year: 2020, Volume: 13, Issue: 13, Pages: 1412-1422

Original Article

Genetic diversity and toxic genes analysis of vibrio spp. isolated from white leg shrimp and marine fishes cultured in Tam G iang lagoon in Thua Thien Hue province, Vietnam

Hoang Tan Quang¹, Tran Thuy Lan¹, Truong Thi Hong Hai¹, Pham Thi Hai Yen², Tran Quang Khanh Van², Ho Thi Tung², Mac Nhu Binh², Nguyen Khoa Huy Son², Nguyen Quang Linh³, Nguyen Duy Quynh Tram^2*

¹Institute of Biotechnology, Hue University, Hue, 530000, Vietnam
²University of Agriculture and Forestry, Hue University, Hue, 530000, Vietnam
³Hue University, Hue, 530000, Vietnam

^*Corresponding author
Nguyen Duy Quynh Tram
University of Agriculture and Forestry, Hue University, Hue, 530000, Vietnam
Email: [email protected]

Received Date:16 April 2020, Accepted Date:23 April 2020, Published Date:23 May 2020

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objective: This study was done to report the results of genetic diversity and toxic genes analysis of Vibrio pathogen isolated from white leg shrimp and marine fishes cultured in Thua Thien Hue province, Vietnam. Methods/statistical analysis: Pathogen Vibrio spp. were isolated from shrimps and fishes, and were identified by 16S rRNA sequencing. The presence of toxin genes in Vibrio spp. strains were determined through the presence of genes encoding toxic proteins (pirAvp, pir-Bvp, tlh, tdh and trh) based on specific primers for these genes. Genetic diversity of Vibrio strains was analysed by RAPD technique. Findings: A total of 120 Vibrio colonies from shrimps (with Acute Hepatopancreatic Necrosis Disease) and fishes (with hemorrhagic disease) cultured in Tam Giang lagoon in Thua Thien Hue, Vietnam were isolated. Of which, 14/54 strains from shrimps had pirAvp and pirBvp genes and 18/66 strains from fishes had tlh gene, and none of Vibrio strains had tdh and trh genes. Randomly amplified polymorphic DNA (RAPD) analysis of 36 Vibrio samples showed the 148 polymorphic DNA fragments from ten random primers. The genetic diversity is high within studied species. In there, V. parahaemolyticus has the highest diversity level (h=0.1645 and I=0.2695) while V. shilonii is lowest (h=0.0136) and I=0.0207). The degree of genetic differentiation among species is also high (Gst=0.4827). Genetic identity between V. parahaemolyticus and V. vulnificus (0.9545) is highest while between V. shilonii and V. harveyi (0.7416) is lowest. The dendrogram also showed that V. parahaemolyticus is closely related to V. vulnificus whereas V. shilonii and V. harveyi have large distance. Application/improvements: This study is aimed to provide scientific data as the basis for the study and production of rapid diagnostic kits in the future.

Keywords: AHPND; Genetic diversity; RAPD; toxic genes; Vibrio; Vietnam

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Copyright

Copyright: © 2020 Quang, Lan, Hai, Yen, Van, Tung, Binh, Son, Linh, Tram. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Published By Indian Society for Education and Environment (iSee)