Indian Journal of Science and Technology
Year: 2011, Volume: 4, Issue: 12, Pages: 1654-1656
Farahnaz Motamedi Sedeh1,2, Hoorieh Soleimanjahi1* , Amirreza Jalilian2 and Homayoon Mahravani3
1 Tarbiat Modares University, Faculty of Medical Science, Tehran, Iran
2 Nuclear Science and Technology Research Institute, Tehran, Iran
3 Razi of Vaccine and Serum Research Institute, Karaj, Iran
[email protected] ; [email protected] *; [email protected] ; [email protected]
Foot and Mouth Disease Virus (FMDV) is a vesicular and contagious disease of cloven-hoofed animals. This study is aimed to inactivate FMD Virus type O/2007/IRN particles by gamma irradiation with unaltered antigenicity. FMD Virus type O/2007/IRN was propagated on BHK21 cells. The virus titration was calculated by TCID50 method. A 60Co-gamma cell instrument Nordian, model 220 with dose rate 4.8 Gy/sec and activity of 20469 Ci was used for the inactivation of the frozen FMD virus samples. Safety test was done by cell culture method, as well as antigenicity of irradiated and non-irradiated virus samples were evaluated by Complement Fixation Test. Irradiated and non-irradiated FMDV particles were concentrated by centrifugation and studied under electron microscopy. The virus titration decreased gradually by increasing of gamma ray doses, according to the Dose/Survival curve for irradiated samples. The D10 value factor (dose of gamma ray which can decrease 90% of virus population) and the optimum dose of gamma ray for FMDV type O/2007/IRN inactivation and unaltered antigenicity was obtained 5-5.5 kGy and 45-50 kGy, respectively. The irradiated inactivated FMD virus with unaltered antigenicity can be used as candidate radio-vaccine with superior safety.
Keywords: Foot and Mouth Disease Virus, Gamma irradiation, Inactivation,
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