Indian Journal of Science and Technology

Abstract

Nanostructure of S-layer forms the monomolecular outermost protein layer in bacteria and archaea that credited with protein or glycoprotein subunits and has crystalline biopolymer structure. This layer protects bacteria to phagocytosis and prohibits the entry of some molecules e.g. antibiotics. The premises of hospital environment can serve as reservoirs of potential pathogens. The goal of study to was investigate the Nanostructure of Surface layer among the Bacillus cereus isolates from hospital environment. The research was performed with laboratory method during 2005- 2007 from Azzahra Hospital and Esfahan University (Isfahan province of Iran). Totally, 194 samples were collected from hospital surface. Environmental samples collected with swab in Nutrient Broth (NB). Bacterial identification was performed based on Bacteriological standard methods using selective culture medium. Samples, cultured in TSA for 16 h, in aerobic condition were then separated for surface proteins and subjected to electrophoresis along with molecular weight marker. S-Layer in B. cereus has 97 KD of molecular weight. From 194 bacterial isolates among hospital environment, the frequency of Bacillus sp. was 26.29%. Among 13 B. cereus strains from hospital environment, only one strain (7.69%) was the producer of S–layer. Result showed that the prevalence B. cereus strain with S-layer in hospital sensitive environment is due to increased antibiotic resistance for nosocomial infection and it is necessary to continue the reduction of transfer of virulence agent and antibiotic resistant in pathogen bacteria. Keywords: Nanostructure of Surface Layer, Bacillus cereus, Antibiotic Resistant, Hospital Environment