• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2009, Volume: 2, Issue: 11, Pages: 20-22

Original Article

Sorting out of interference in detection of endotoxins in biotherapeutic drugs


The effect of varying interference factors responsible for false positive results was investigated. The interfering factors inhibiting the endotoxins in activated lymphocytes sample was sorted by heat denaturation. These activated lymphocytes are biotherapeutic drugs which are used for autologous immune enhancement therapy. The technique used for denaturation can be applied to protein samples as well, to get rid of inhibition while quantifying endotoxins.

Keywords: LAL, CSE, Bacterial Endotoxin, Endotoxin limit and Interference. 


Subscribe now for latest articles and news.