Sorting out of interference in detection of endotoxins in biotherapeutic drugs

Ramesh Pennamareddy; K  Prabakar    and J  Pandiyan

doi:10.17485/ijst/2009/v2i11.13

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Sorting out of interference in detection of endotoxins in biotherapeutic drugs

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2009/v2i11.13

Year: 2009, Volume: 2, Issue: 11, Pages: 20-22

Original Article

Sorting out of interference in detection of endotoxins in biotherapeutic drugs

Ramesh Pennamareddy¹ , K. Prabakar¹ and J. Pandiyan²

¹P.G. Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli;
²P.G. Research Dept. of Zoology & Wildlife Biology, AVC College (Autonomous), Mannampandal-609 305, TN, India

*Author for the correspondence:
J. Pandiyan
P.G. Research Dept. of Zoology & Wildlife Biology,
AVC College (Autonomous), Mannampandal-609 305, TN, India
E-mail: [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The effect of varying interference factors responsible for false positive results was investigated. The interfering factors inhibiting the endotoxins in activated lymphocytes sample was sorted by heat denaturation. These activated lymphocytes are biotherapeutic drugs which are used for autologous immune enhancement therapy. The technique used for denaturation can be applied to protein samples as well, to get rid of inhibition while quantifying endotoxins.

Keywords: LAL, CSE, Bacterial Endotoxin, Endotoxin limit and Interference.