• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2023, Volume: 16, Issue: Special Issue 1, Pages: 48-56

Original Article

Synergistic Antioxidative Potential of Cosmosiin and Vanillic Acid in vitro and ex vivo

Received Date:23 January 2023, Accepted Date:09 June 2023, Published Date:30 July 2023


Objectives: To determine the synergistic antioxidative potential of cosmosiin and vanillic acid. Methods: The synergistic antioxidative potential of cosmosiin and vanillic acid was evaluated using their ability to scavenge 2, 2′- azino-bis-(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide anions (O2  􀀀), and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). The ability to convert Fe3+ into Fe2+ was used to evaluate the combined reducing power of cosmosiin and vanillic acid. The synergistic effect of cosmosiin and vanillic acid was also tested in an ex vivo setting using mouse erythrocytes and livers, respectively, for their anti-haemolytic activity and inhibitory effects on lipid peroxidation. Findings: When compared to the individual compound, the mixture of cosmosiin and vanillic acid exhibited higher scavenging activities for DPPH, ABTS and O2  􀀀 with IC50 of 112.0  0.46 mM, 126.17  4.97 mM, and 5.14  0.18 mM, respectively. Consistently, the combination of cosmosiin and vanillic acid possessed higher reducing power than either compound alone. A mixture of cosmosiin and vanillic acid also showed higher inhibitory activities against mice erythrocyte hemolysis and lipid peroxidation in the liver homogenate with an inhibition rate of 51 % and 90% respectively. Novelty: Our study corroborates that cosmosiin and vanillic acid have stronger antioxidative effects when used in combination than when used individually.

Keywords: Cosmosin; Vanillic acid; Free radical scavenging activity; Antioxidant; In vitro


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© 2023 Lalfakawmi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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