Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue

Dang Thanh Long; Van Thi My Le; Hoang Tan Quang; Hoang Thi Ngoc Han; Tong Thi Hue; Nguyen Thi Qu   nh Trang

doi:10.17485/IJST/v16i44.2434

Article

Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue

VIEWS 289
PDF 56

Indian Journal of Science and Technology

DOI: 10.17485/IJST/v16i44.2434

Year: 2023, Volume: 16, Issue: 44, Pages: 4114-4122

Original Article

Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue

Dang Thanh Long^1*, Van Thi My Le², Hoang Tan Quang¹, Hoang Thi Ngoc Han³, Tong Thi Hue¹, Nguyen Thi Quỳnh Trang⁴

¹Institute of Biotechnology, Hue University, Hue city, Thua Thien Hue, 49000, Vietnam
²University of Sciences, Hue University, Hue city, Thua Thien Hue, 49000, Vietnam
³Department of Natural resources and Environment of Quang Tri, Dong Ha city, Quang Tri province, Vietnam
⁴University of Education, Hue University, Hue city, Thua Thien Hue, 49000, Vietnam

*Corresponding Author
Email: [email protected]

Received Date:24 September 2023, Accepted Date:16 October 2023, Published Date:29 November 2023

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives : In this study, we investigate the bioactive potential of Helicteres hirsuta Lour. (H. hirsuta L.). Methods: H. hirsuta collected in Thua Thien Hue province was used. The methanolic extract of roots and leaves was studied for its biochemical components based on the High-Performance Liquid Chromatography method and also tested for antioxidant activities Mention how the test was carried out and the endpoints considered. Findings : The results show that some biochemical components contained in the dry materials of H. hirsuta leaves and roots are safe and suitable, allowing use in the extraction and collection of total flavonoids. The optimal extraction conditions using the ultrasonic bath method included a time of 50 min, a material/solvent ratio of 1:20 (g/mL), ultrasonic frequency (12 Hz), and a solvent concentration of methanol of 50% (v/v). In this condition, the highest total flavonoid content obtained from H. hirsuta leaves and roots were 0.841 mg CE/mL extract (leaf) and 0.416 mg CE/mL extract (root), respectively. The DPPH free radical scavenging ability at 50% concentration (IC50) of leaf and root extracts varied with IC50 of 0.778 mL and 0.433 mL, respectively. The methanol extract from the leaf and root of H. hirsuta has the best inhibitory effect on the human liver cancer cell line Hep-G2 (IC50 leaf = 177.22 µg/mL, and IC50 root = 165.21 µg/mL). Novelty: The results of this study are valuable reference information for exploiting, using, and processing products from H. hirsuta trees collected in Thua Thien Hue in the future.

Keywords: Flavonoid, Helicteres hirsuta Lour., Flavonoid, Extract, Leaf, Root

References

Pham HNT, Nguyen VT, Vuong QV, Bowyer MC, Scarlett CJ. Bioactive Compound Yield and Antioxidant Capacity of Helicteres hirsuta Lour. Stem as Affected by Various Solvents and Drying Methods. Journal of Food Processing and Preservation. 2017;41(1):e12879. Available from: https://doi.org/10.1111/jfpp.12879
Vijayan K, Tsou CH. DNA barcoding in plants: taxonomy in a new perspective. Current Science. 2010;99:1530–1540. Available from: https://www.jstor.org/stable/24069450
Jain A, Sinha P, Desai NS. Estimation of flavonoid, phenol content and antioxidant potential of Indian screw tree Helicteres isora L. International Journal of Pharma Sciences and Research. 2014;5(4):1320–1330. Available from: http://dx.doi.org/10.13040/IJPSR.0975-8232.5(4).1320-30
Pham H, Nguyen VT, Vuong QV, Bowyer MC, Scarlett CJ. Effect of Extraction Solvents and Drying Methods on the Physicochemical and Antioxidant Properties of Helicteres hirsuta Lour. Leaves. Technologies. 2015;3(4):285–301. Available from: https://doi.org/10.3390/technologies3040285
Hieu LT, Son LL, Vu H, Hoa D, Thi T. Antioxidant activity of extracts from Helicteres hirsuta Lour. Fruits in Thua Thien Hue province. Journal of Science and Technology. 2019;14(2):1–9.
Loganayaki N, Siddhuraju P, Manian S. Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L. Journal of Food Science and Technology. 2013;50(4):687–695. Available from: https://doi.org/10.1007/s13197-011-0389-x
Yen L, Ha N, Nga NT, Binh TV, Oanh TT. Anti-inflammatory effects and analgesic effects of liquid extracts from Helicteres hirsuta Lour. in Swiss mice. Journal of medicine and pharmacy. 2017;496:56–59.
Duyen NH, Phuoc LT. Survey of chemicalcomposition and toxicityactivity in Hep-G2 cell of Helicteres hirsuta L. Can Tho University Journal of Science. 2016;47:93–97. Available from: https://doi.org/10.22144/jvn.2016.605
Pham HNT, Vuong QV, Bowyer MC, Scarlett CJ. In vitro anti-pancreatic cancer activity of HPLC-derived fractions from Helicteres hirsuta Lour. stem. Molecular Biology Reports. 2020;47(2):897–905. Available from: https://doi.org/10.1007/s11033-019-05180-0
Pham HNT, Vuong QV, Bowyer MC, Scarlett CJ. Phytochemical Profiles and Potential Health Benefits of Helicteres hirsuta Lour. The 1st International Electronic Conference on Food Science and Functional Foods. 2021;70:43. Available from: https://doi.org/10.3390/foods_2020-07804
Dinda B, Debnath S, Harigaya Y. Naturally Occurring Iridoids. A Review, Part 1. Chemical and Pharmaceutical Bulletin. 2007;55(2):159–222. Available from: https://doi.org/10.1248/cpb.55.159
Pham HNT, Vuong QV, Bowyer MC, Scarlett CJ. Optimum conventional extraction conditions for phenolics, flavonoids, and antioxidant capacity of <i>Helicteres hirsuta</i> Lour. Asia-Pacific Journal of Chemical Engineering. 2017;12(2):332–347. Available from: https://doi.org/10.1002/apj.2076
Quang DN, Pham CT, Le LTK, Ta QN, Dang NK, Hoang NT, et al. Cytotoxic constituents from Helicteres hirsuta collected in Vietnam. Natural Product Research. 2020;34(4):585–589. Available from: https://doi.org/10.1080/14786419.2018.1490907
Chin YWW, Jones WP, Rachman I, Riswan S, Kardono LBS, Chai HBB, et al. Cytotoxic lignans from the stems ofHelicteres hirsuta collected in indonesia. Phytotherapy Research. 2006;20(1):62–65. Available from: https://doi.org/10.1002/ptr.1806
Hong P. Biochemical engineering. 2003.
Vuong QV, Hirun S, Roach PD, Bowyer MC, Phillips PA, Scarlett CJ. Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts. Journal of Herbal Medicine. 2013;3(3):104–111. Available from: https://doi.org/10.1016/j.hermed.2013.04.004
Long DT, Hong H, Trang N, Tram L, Tien N, Hanh N. Study of the Procedure for Bath Ultrasound-Assisted Extraction of Total Flavonoid from Lotus Seeds and Testing Some Biological Activities. Indian Journal of Agricultural Research. 2020;54(5). Available from: https://doi.org/10.18805/IJARe.A-525
Marxen K, Vanselow K, Lippemeier S, Hintze R, Ruser A, Hansen UPP. Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements. Sensors. 2007;7(10):2080–2095. Available from: https://doi.org/10.3390/s7102080
Van CV. Dictionary of Vietnamese medicinal plants. Medical Publishing. 1997.
Tra NT, Cham BT, Ha NTT, Anh LTT, Tuyen NV, Son NT. Antioxidant Constituents from the Stem and Leaf of Helicteres hirsuta Loureiro. The Natural Products Journal. 2021;11(5):592–599. Available from: https://doi.org/10.2174/2210315510999200728131328
Pham HNT, Vuong QV, Bowyer MC, Scarlett CJ. Phytochemical Profiles and Potential Health Benefits of Helicteres hirsuta Lour. The 1st International Electronic Conference on Food Science and Functional Foods. 2021;70:43. Available from: https://doi.org/10.3390/foods_2020-07804
Hieu LT, Thi TTV, Son LL, Nhung NM, Diep HTN, Mechler A, et al. Phenolic Contents and Antioxidant Activity of Helicteres Hirsuta Extracts. Letters in Organic Chemistry. 2021;18(2):128–133. Available from: https://doi.org/10.2174/1570178617999200728205824
Thuy P. In vitro antioxidant and cytotoxic activities of alcohol and chloroform extract Helicteres hirsuta Lour. Sterculiaceae. Journal of Science and Technology. 2018;4:90–92.

Copyright

© 2023 Long et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)