Evaluation of phytochemical constituents and hepatoprotective effects of Physcia grisea in albino rats

Martina Chinagorom Onu; Emmanuel Ikechukwu Eze

doi:10.17485/IJST/v14i12.1990

Article

Evaluation of phytochemical constituents and hepatoprotective effects of Physcia grisea in albino rats

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Indian Journal of Science and Technology

DOI: 10.17485/IJST/v14i12.1990

Year: 2021, Volume: 14, Issue: 12, Pages: 1006-1012

Original Article

Evaluation of phytochemical constituents and hepatoprotective effects of Physcia grisea in albino rats

Martina Chinagorom Onu¹, Emmanuel Ikechukwu Eze^2*

¹Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka
²Department of Crop Science, University of Nigeria, Nsukka. Tel.: 08063290853

*Corresponding Author
Tel: 08063290853
Email: [email protected]

Received Date:16 November 2020, Accepted Date:26 January 2021, Published Date:14 April 2021

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: The study aimed to evaluate the phytochemical constituents and
hepatoprotective effects of Physcia grisea in albino rats. Methods/statisticalanalysis:The analysis of phytochemical constituents of P. grisea was carried out to determine the level of tannins, phenols, terpenes, flavonoids and alkaloids present in the sample. The blood samples from four groups (A, B, C, and D) of albino rats with each group containing four per group were analyzed for Aspartate aminotransferase (AST) and bilirubin secretions which are two early indicators of liver injury. The analysis was carried out in two phases; phase 1 and phase 2. In phase I, which was regarded as the base line analysis, the weights of the animals were taken and the blood samples were collected following ocular puncture. In the second phase of the analysis, the group A, B and C were administered 500 mg/kg, 250 mg/kg and 125 mg/kg concentrations of P. grisea respectively while the control group was administered only tragecant solution through the oral route. After 3 weeks of the administration, their blood samples were collected for another analysis using the same method of blood collection. The data obtained were analyzed using GenStat Release 10.3DE (2011) statistical software. Findings: The results showed varying levels of phytochemicals in P. grisea, alkaloids (36.6%), flavonoids (3.6 %), saponins (2.7 %), total phenols (25.15 %), tannins (0.029 %) and terpenes (2.0 %). The results of the in-vivo studies showed that P. grisea had no significant effect on the AST and bilirubin secretions at 125mg/kg after 3 weeks of the administration. This was an indication that there was no liver injury at this dose within the period of administration. Application/improvements: If properly harnessed, P. grisea extract could provide a novel and cheap source of medicine for treatment of liver diseases resulting from AST and bilirubin secretions.

Keywords: Phytochemical; Physea grisea; Aspertate Aminotransferase; Bilirubin

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© 2021 Onu & Eze.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)