Isolation and characterization of probiotic Bacillus subtilis SK09 from dairy effluent

G  Sreekumar  and Soundarajan Krishnan

doi:10.17485/ijst/2010/v3i8.13

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Isolation and characterization of probiotic Bacillus subtilis SK09 from dairy effluent

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2010/v3i8.13

Year: 2010, Volume: 3, Issue: 8, Pages: 863-866

Original Article

Isolation and characterization of probiotic Bacillus subtilis SK09 from dairy effluent

G. Sreekumar¹ and Soundarajan Krishnan^2*

¹ Department of Biotechnology, St. Joseph’s College of Engineering, Chennai-600119, India² Department of Chemical Engineering, Sathyabama University, Chennai-600119, India
[email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Health promoting microorganisms such as probiotics are recently been used as food additive and therapeutic supplement especially as enhancer of prophylaxis and digestion. Here we report the isolation and characterization of a probiotic bacillus species, with the ability to ferment lactose, from dairy effluent. Biochemical test and 16S rRNA sequencing were done in order to establish the species and strain details. The species was found to be Bacillus subtilis SK09 with a unique ability to ferment lactose. The strain was also subjected to commonly available antibiotics for its antibiotic susceptibility for its probiotic credibility.
Keywords: Probiotic bacteria, Bacillus subtilis, lactose intolerance, 16SrRNA sequencing.